2efw

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{{Seed}}
 
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[[Image:2efw.png|left|200px]]
 
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==Crystal structure of the RTP:nRB complex from Bacillus subtilis==
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The line below this paragraph, containing "STRUCTURE_2efw", creates the "Structure Box" on the page.
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<StructureSection load='2efw' size='340' side='right'caption='[[2efw]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2efw]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EFW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2efw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2efw OCA], [https://pdbe.org/2efw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2efw RCSB], [https://www.ebi.ac.uk/pdbsum/2efw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2efw ProSAT]</span></td></tr>
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{{STRUCTURE_2efw| PDB=2efw | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RTP_BACSU RTP_BACSU] Plays a role in DNA replication and termination (fork arrest mechanism). Two dimers of rtp bind to the two inverted repeat regions (IRI and IRII) present in the termination site. The binding of each dimer is centered on an 8 bp direct repeat.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In Bacillus subtilis, the termination of DNA replication via polar fork arrest is effected by a specific protein:DNA complex formed between the replication terminator protein (RTP) and DNA terminator sites. We report the crystal structure of a replication terminator protein homologue (RTP.C110S) of B. subtilis in complex with the high affinity component of one of its cognate DNA termination sites, known as the TerI B-site, refined at 2.5 A resolution. The 21 bp RTP:DNA complex displays marked structural asymmetry in both the homodimeric protein and the DNA. This is in contrast to the previously reported complex formed with a symmetrical TerI B-site homologue. The induced asymmetry is consistent with the complex's solution properties as determined using NMR spectroscopy. Concomitant with this asymmetry is variation in the protein:DNA binding pattern for each of the subunits of the RTP homodimer. It is proposed that the asymmetric "wing" positions, as well as other asymmetrical features of the RTP:DNA complex, are critical for the cooperative binding that underlies the mechanism of polar fork arrest at the complete terminator site.
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===Crystal structure of the RTP:nRB complex from Bacillus subtilis===
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An asymmetric structure of the Bacillus subtilis replication terminator protein in complex with DNA.,Vivian JP, Porter CJ, Wilce JA, Wilce MC J Mol Biol. 2007 Jul 13;370(3):481-91. Epub 2007 Mar 2. PMID:17521668<ref>PMID:17521668</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2efw" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17521668}}, adds the Publication Abstract to the page
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*[[Replication Termination Protein|Replication Termination Protein]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17521668 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17521668}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2EFW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EFW OCA].
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==Reference==
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An asymmetric structure of the Bacillus subtilis replication terminator protein in complex with DNA., Vivian JP, Porter CJ, Wilce JA, Wilce MC, J Mol Biol. 2007 Jul 13;370(3):481-91. Epub 2007 Mar 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17521668 17521668]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Porter, C J.]]
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[[Category: Porter CJ]]
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[[Category: Vivian, J P.]]
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[[Category: Vivian JP]]
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[[Category: Wilce, J A.]]
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[[Category: Wilce JA]]
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[[Category: Wilce, M C.J.]]
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[[Category: Wilce MCJ]]
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[[Category: Winged -helix protein]]
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[[Category: Dna replication termination]]
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[[Category: Protein-dna complex]]
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[[Category: Replication fork arrest]]
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[[Category: Replication/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 06:52:31 2008''
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Current revision

Crystal structure of the RTP:nRB complex from Bacillus subtilis

PDB ID 2efw

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