1pg4

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(New page: 200px<br /><applet load="1pg4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pg4, resolution 1.75&Aring;" /> '''Acetyl CoA Synthetas...)
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[[Image:1pg4.gif|left|200px]]<br /><applet load="1pg4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pg4, resolution 1.75&Aring;" />
 
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'''Acetyl CoA Synthetase, Salmonella enterica'''<br />
 
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==Overview==
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==Acetyl CoA Synthetase, Salmonella enterica==
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Acetyl-coenzyme A synthetase catalyzes the two-step synthesis of, acetyl-CoA from acetate, ATP, and CoA and belongs to a family of, adenylate-forming enzymes that generate an acyl-AMP intermediate. This, family includes other acyl- and aryl-CoA synthetases, firefly luciferase, and the adenylation domains of the modular nonribosomal peptide, synthetases. We have determined the X-ray crystal structure of acetyl-CoA, synthetase complexed with adenosine-5'-propylphosphate and CoA. The, structure identifies the CoA binding pocket as well as a new conformation, for members of this enzyme family in which the approximately 110-residue, C-terminal domain exhibits a large rotation compared to structures of, peptide synthetase adenylation domains. This domain movement presents a, new set of residues to the active site and removes a conserved lysine, residue that was previously shown to be important for catalysis of the, adenylation half-reaction. Comparison of our structure with kinetic and, structural data of closely related enzymes suggests that the members of, the adenylate-forming family of enzymes may adopt two different, orientations to catalyze the two half-reactions. Additionally, we provide, a structural explanation for the recently shown control of enzyme activity, by acetylation of an active site lysine.
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<StructureSection load='1pg4' size='340' side='right'caption='[[1pg4]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1nnm 1nnm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PG4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRX:ADENOSINE-5-MONOPHOSPHATE-PROPYL+ESTER'>PRX</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pg4 OCA], [https://pdbe.org/1pg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pg4 RCSB], [https://www.ebi.ac.uk/pdbsum/1pg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pg4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACSA_SALTY ACSA_SALTY] Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. Acs undergoes a two-step reaction. In the first half reaction, Acs combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.<ref>PMID:17497934</ref> Enables the cell to use acetate during aerobic growth to generate energy via the TCA cycle, and biosynthetic compounds via the glyoxylate shunt. Acetylates CheY, the response regulator involved in flagellar movement and chemotaxis (By similarity).<ref>PMID:17497934</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pg/1pg4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pg4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica] with CL, MG, COA, PRX and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. This structure superseeds the now removed PDB entry 1NNM. Active as [http://en.wikipedia.org/wiki/Acetate--CoA_ligase Acetate--CoA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.2.1.1 6.2.1.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PG4 OCA].
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*[[Acetyl-CoA synthetase 3D structures|Acetyl-CoA synthetase 3D structures]]
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== References ==
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==Reference==
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<references/>
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The 1.75 A crystal structure of acetyl-CoA synthetase bound to adenosine-5'-propylphosphate and coenzyme A., Gulick AM, Starai VJ, Horswill AR, Homick KM, Escalante-Semerena JC, Biochemistry. 2003 Mar 18;42(10):2866-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12627952 12627952]
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__TOC__
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[[Category: Acetate--CoA ligase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Salmonella enterica]]
[[Category: Salmonella enterica]]
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[[Category: Single protein]]
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[[Category: Escalante-Semerena JC]]
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[[Category: Escalante-Semerena, J.C.]]
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[[Category: Gulick AM]]
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[[Category: Gulick, A.M.]]
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[[Category: Homick KM]]
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[[Category: Homick, K.M.]]
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[[Category: Horswill AR]]
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[[Category: Horswill, A.R.]]
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[[Category: Starai VJ]]
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[[Category: Starai, V.J.]]
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[[Category: CL]]
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[[Category: COA]]
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[[Category: EDO]]
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[[Category: MG]]
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[[Category: PRX]]
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[[Category: amp-forming; adenylate-forming; thioester-forming]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:46:44 2007''
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Current revision

Acetyl CoA Synthetase, Salmonella enterica

PDB ID 1pg4

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