1pga

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(New page: 200px<br /><applet load="1pga" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pga, resolution 2.07&Aring;" /> '''TWO CRYSTAL STRUCTUR...)
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[[Image:1pga.jpg|left|200px]]<br /><applet load="1pga" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pga, resolution 2.07&Aring;" />
 
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'''TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON WITH NMR'''<br />
 
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==Overview==
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==TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON WITH NMR==
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The structure of the 56-residue B1 immunoglobulin-binding domain from, streptococcal protein G has been determined in two different crystal, forms. The crystal structures were deduced by molecular replacement, based, on the structure of the B2 domain (Brookhaven accession code 1PGX). Final, R values are 0.174 and 0.198 for orthorhombic and trigonal forms, for, diffraction data from 6.0 to 2.07 A and from 6 to 1.92 A, respectively., The orthorhombic crystals have an unusually high packing density for, protein crystals, with Vm = 1.66 and a solvent content of 26%. The protein, structure is found to be very similar (rms deviation 0.25 A for 56 C, alpha's) in the two crystal forms, with an efficiently packed hydrophobic, core between a four-stranded beta-sheet and a four-turn alpha-helix. The, B1 domain has the same fold and general structure as the B2 domain (rms, deviations 0.36 and 0.39 A), despite the six residue differences between, them. The crystallographic models differ from NMR-derived models in, several local regions, primarily in the loop involving residues 46-51;, other significant variations are observed in the helix and in the, structure of bound water. The primary crystal contact is the same in both, crystal forms, involving both sheet edges to form extended beta-sheets, throughout the crystals.
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<StructureSection load='1pga' size='340' side='right'caption='[[1pga]], [[Resolution|resolution]] 2.07&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pga]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_sp._GX7805 Streptococcus sp. GX7805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PGA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.07&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pga OCA], [https://pdbe.org/1pga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pga RCSB], [https://www.ebi.ac.uk/pdbsum/1pga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pga ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPG1_STRSG SPG1_STRSG] Binds to the constant Fc region of IgG with high affinity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pg/1pga_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pga ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PGA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._gx7805 Streptococcus sp. gx7805]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PGA OCA].
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*[[Protein G|Protein G]]
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__TOC__
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==Reference==
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</StructureSection>
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Two crystal structures of the B1 immunoglobulin-binding domain of streptococcal protein G and comparison with NMR., Gallagher T, Alexander P, Bryan P, Gilliland GL, Biochemistry. 1994 Apr 19;33(15):4721-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8161530 8161530]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Streptococcus sp. GX7805]]
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[[Category: Streptococcus sp. gx7805]]
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[[Category: Alexander P]]
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[[Category: Alexander, P.]]
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[[Category: Bryan P]]
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[[Category: Bryan, P.]]
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[[Category: Gallagher T]]
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[[Category: Gallagher, T.]]
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[[Category: Gilliland GL]]
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[[Category: Gilliland, G.L.]]
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[[Category: immunoglobulin binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:46:58 2007''
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TWO CRYSTAL STRUCTURES OF THE B1 IMMUNOGLOBULIN-BINDING DOMAIN OF STREPTOCOCCAL PROTEIN G AND COMPARISON WITH NMR

PDB ID 1pga

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