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1pil

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(New page: 200px<br /><applet load="1pil" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pil, resolution 2.7&Aring;" /> '''STRUCTURE OF THE ESCH...)
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[[Image:1pil.gif|left|200px]]<br /><applet load="1pil" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pil, resolution 2.7&Aring;" />
 
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'''STRUCTURE OF THE ESCHERICHIA COLI SIGNAL TRANSDUCING PROTEIN PII'''<br />
 
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==Overview==
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==STRUCTURE OF THE ESCHERICHIA COLI SIGNAL TRANSDUCING PROTEIN PII==
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BACKGROUND: In Gram-negative proteobacteria, the nitrogen level in the, cell is reflected by the uridylylation status of a key signal transducing, protein, PII. PII modulates the activity of glutamine synthetase (GS), through its interaction with adenylyl transferase and it represses the, expression of GS by acting in concert with nitrogen regulatory protein II., RESULTS: The three-dimensional structure of the Escherichia coli PII, trimer has been determined at 2.7 A resolution. PII shows a low level of, structural similarity to a broad family of alpha/beta proteins and, contains a double beta alpha beta motif. The PII trimer contains three, beta-sheets, each of which is composed of strands from each of the three, monomers. These are surrounded by six alpha-helices. CONCLUSIONS: The, structure of PII suggests potential regions of interaction with other, proteins and serves as an initial step in understanding its signal, transducing role in nitrogen regulation.
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<StructureSection load='1pil' size='340' side='right'caption='[[1pil]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1pil]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PIL FirstGlance]. <br>
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1PIL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PIL OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pil OCA], [https://pdbe.org/1pil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pil RCSB], [https://www.ebi.ac.uk/pdbsum/1pil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pil ProSAT]</span></td></tr>
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==Reference==
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</table>
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Structure of the Escherichia coli signal transducing protein PII., Cheah E, Carr PD, Suffolk PM, Vasudevan SG, Dixon NE, Ollis DL, Structure. 1994 Oct 15;2(10):981-90. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7866749 7866749]
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== Function ==
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[https://www.uniprot.org/uniprot/GLNB_ECOLI GLNB_ECOLI] P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of GlnA. When P-II is uridylylated to P-II-UMP, these events are reversed. When the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP, which causes the deadenylation of glutamine synthetase by GlnE, so activating the enzyme.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pi/1pil_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pil ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Carr, P.D.]]
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[[Category: Carr PD]]
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[[Category: Cheah, U.E.]]
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[[Category: Cheah UE]]
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[[Category: Ollis, D.L.]]
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[[Category: Ollis DL]]
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[[Category: Suffolk, P.M.]]
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[[Category: Suffolk PM]]
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[[Category: nitrogen regulatory protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:50:30 2007''
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Current revision

STRUCTURE OF THE ESCHERICHIA COLI SIGNAL TRANSDUCING PROTEIN PII

PDB ID 1pil

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