1pio

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(New page: 200px<br /><applet load="1pio" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pio, resolution 2.8&Aring;" /> '''AN ENGINEERED STAPHYL...)
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[[Image:1pio.jpg|left|200px]]<br /><applet load="1pio" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pio, resolution 2.8&Aring;" />
 
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'''AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS'''<br />
 
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==Overview==
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==AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS==
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The beta-lactamase from Staphylococcus aureus PC1 has been cloned into an, Escherichia coli vector for site-directed mutagenesis and high-level, protein expression. A mutant enzyme has been produced in which Ala238 is, replaced by a serine, and Ile239 is deleted (A238S:I239del). The, engineered enzyme hydrolyses third-generation cephalosporins substantially, more rapidly than the parental enzyme does, while hydrolysis of, benzylpenicillin is slower with the mutant than with the wild-type and, native enzymes. The mutant beta-lactamase has been crystallized and the, structure determined and refined at 2.8 A resolution. The disposition of, the beta-strand which forms the side of the active site is altered in, comparison with the native S. aureus beta-lactamase structure, widening, the active site cleft and providing space to accommodate the bulky, side-chains of the third-generation cephalosporins.
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<StructureSection load='1pio' size='340' side='right'caption='[[1pio]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pio]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PIO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PIO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pio FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pio OCA], [https://pdbe.org/1pio PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pio RCSB], [https://www.ebi.ac.uk/pdbsum/1pio PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pio ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BLAC_STAAU BLAC_STAAU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pi/1pio_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pio ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The beta-lactamase from Staphylococcus aureus PC1 has been cloned into an Escherichia coli vector for site-directed mutagenesis and high-level protein expression. A mutant enzyme has been produced in which Ala238 is replaced by a serine, and Ile239 is deleted (A238S:I239del). The engineered enzyme hydrolyses third-generation cephalosporins substantially more rapidly than the parental enzyme does, while hydrolysis of benzylpenicillin is slower with the mutant than with the wild-type and native enzymes. The mutant beta-lactamase has been crystallized and the structure determined and refined at 2.8 A resolution. The disposition of the beta-strand which forms the side of the active site is altered in comparison with the native S. aureus beta-lactamase structure, widening the active site cleft and providing space to accommodate the bulky side-chains of the third-generation cephalosporins.
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==About this Structure==
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An engineered Staphylococcus aureus PC1 beta-lactamase that hydrolyses third-generation cephalosporins.,Zawadzke LE, Smith TJ, Herzberg O Protein Eng. 1995 Dec;8(12):1275-85. PMID:8869640<ref>PMID:8869640</ref>
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1PIO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Active as [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PIO OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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An engineered Staphylococcus aureus PC1 beta-lactamase that hydrolyses third-generation cephalosporins., Zawadzke LE, Smith TJ, Herzberg O, Protein Eng. 1995 Dec;8(12):1275-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8869640 8869640]
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</div>
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[[Category: Beta-lactamase]]
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<div class="pdbe-citations 1pio" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
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[[Category: Herzberg, O.]]
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[[Category: Zawadzke, L.E.]]
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[[Category: hydrolase (acting on cyclic amides)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:50:39 2007''
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==See Also==
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Staphylococcus aureus]]
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[[Category: Herzberg O]]
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[[Category: Zawadzke LE]]

Current revision

AN ENGINEERED STAPHYLOCOCCUS AUREUS PC1 BETA-LACTAMASE THAT HYDROLYSES THIRD GENERATION CEPHALOSPORINS

PDB ID 1pio

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