1upt

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{{Seed}}
 
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[[Image:1upt.png|left|200px]]
 
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==Structure of a complex of the golgin-245 GRIP domain with Arl1==
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The line below this paragraph, containing "STRUCTURE_1upt", creates the "Structure Box" on the page.
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<StructureSection load='1upt' size='340' side='right'caption='[[1upt]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1upt]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UPT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_1upt| PDB=1upt | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1upt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1upt OCA], [https://pdbe.org/1upt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1upt RCSB], [https://www.ebi.ac.uk/pdbsum/1upt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1upt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARL1_HUMAN ARL1_HUMAN] GTP-binding protein that has very low efficiency as allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Can activate phospholipase D with very low efficiency. Important for normal function of the Golgi apparatus.<ref>PMID:9624189</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/up/1upt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1upt ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Golgins are large coiled-coil proteins that play a role in Golgi structure and vesicle traffic. The Arf-like GTPase Arl1 regulates the translocation of GRIP domain-containing golgins to Golgi membranes. We report here the 1.7 A resolution structure of human Arl1-GTP in a complex with the GRIP domain of golgin-245. The structure reveals that the GRIP domain consists of an S-shaped arrangement of three helices. The domain forms a homodimer that binds two Arl1-GTPs using two helices from each monomer. The structure is consistent with golgin-245 forming parallel coiled-coils and suggests how Arl1-GTP/GRIP complexes interact with Golgi membranes via the N termini of Arl1-GTP and the C-terminal tails of the GRIP domains. In cells, bivalent association with Arl1-GTP would increase residence time of the golgins on Golgi membranes. Despite no conservation of sequence, topology, or even helical direction, several other effectors form similar interactions with small GTPases via a pair of alpha helices, suggesting a common structural basis for effector recognition.
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===STRUCTURE OF A COMPLEX OF THE GOLGIN-245 GRIP DOMAIN WITH ARL1===
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Structural basis for Arl1-dependent targeting of homodimeric GRIP domains to the Golgi apparatus.,Panic B, Perisic O, Veprintsev DB, Williams RL, Munro S Mol Cell. 2003 Oct;12(4):863-74. PMID:14580338<ref>PMID:14580338</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_14580338}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1upt" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 14580338 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_14580338}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1UPT is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UPT OCA].
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==Reference==
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Structural basis for Arl1-dependent targeting of homodimeric GRIP domains to the Golgi apparatus., Panic B, Perisic O, Veprintsev DB, Williams RL, Munro S, Mol Cell. 2003 Oct;12(4):863-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14580338 14580338]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Munro, S.]]
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[[Category: Munro S]]
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[[Category: Panic, B.]]
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[[Category: Panic B]]
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[[Category: Perisic, O.]]
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[[Category: Perisic O]]
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[[Category: Veprintsev, D B.]]
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[[Category: Veprintsev DB]]
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[[Category: Williams, R L.]]
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[[Category: Williams RL]]
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[[Category: Arl1]]
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[[Category: G-protein]]
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[[Category: Golgi]]
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[[Category: Golgin]]
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[[Category: Golgin-245]]
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[[Category: Grip]]
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[[Category: Grip dimer]]
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[[Category: Gtpase]]
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[[Category: Protein sorting]]
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[[Category: Vesicle trafficking]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:07:37 2008''
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Current revision

Structure of a complex of the golgin-245 GRIP domain with Arl1

PDB ID 1upt

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