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1pmb

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(New page: 200px<br /><applet load="1pmb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pmb, resolution 2.5&Aring;" /> '''THE DETERMINATION OF ...)
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[[Image:1pmb.gif|left|200px]]<br /><applet load="1pmb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pmb, resolution 2.5&Aring;" />
 
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'''THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT'''<br />
 
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==Overview==
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==THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT==
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As part of a protein engineering study, the X-ray crystal structure of, recombinant pig myoglobin, prepared and crystallized from E. coli, has, been determined. Diffraction data were collected to 2.5 A spacing using a, synchrotron X-ray source. The structure was solved using the, molecular-replacement method and refined using least-squares minimization, procedures to a crystallographic R factor of 18.5% using 14,481, reflections between 10 and 2.5 A. A preliminary comparison of the, structure of pig myoglobin with other myoglobin structures is presented.
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<StructureSection load='1pmb' size='340' side='right'caption='[[1pmb]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pmb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PMB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pmb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pmb OCA], [https://pdbe.org/1pmb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pmb RCSB], [https://www.ebi.ac.uk/pdbsum/1pmb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pmb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYG_PIG MYG_PIG] Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pm/1pmb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pmb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PMB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PMB OCA].
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*[[Myoglobin 3D structures|Myoglobin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Determination of the crystal structure of recombinant pig myoglobin by molecular replacement and its refinement., Smerdon SJ, Oldfield TJ, Dodson EJ, Dodson GG, Hubbard RE, Wilkinson AJ, Acta Crystallogr B. 1990 Jun 1;46 ( Pt 3):370-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2383370 2383370]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Dodson, E.J.]]
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[[Category: Dodson EJ]]
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[[Category: Dodson, G.G.]]
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[[Category: Dodson GG]]
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[[Category: Hubbard, R.E.]]
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[[Category: Hubbard RE]]
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[[Category: Oldfield, T.J.]]
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[[Category: Oldfield TJ]]
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[[Category: Smerdon, S.J.]]
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[[Category: Smerdon SJ]]
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[[Category: Wilkinson, A.J.]]
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[[Category: Wilkinson AJ]]
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[[Category: HEM]]
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[[Category: oxygen storage]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:55:16 2007''
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Current revision

THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT

PDB ID 1pmb

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