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1pmm

From Proteopedia

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(New page: 200px<br /><applet load="1pmm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pmm, resolution 2.00&Aring;" /> '''Crystal structure of...)
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[[Image:1pmm.gif|left|200px]]<br /><applet load="1pmm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pmm, resolution 2.00&Aring;" />
 
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'''Crystal structure of Escherichia coli GadB (low pH)'''<br />
 
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==Overview==
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==Crystal structure of Escherichia coli GadB (low pH)==
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Glutamate decarboxylase is a vitamin B6-dependent enzyme, which catalyses, the decarboxylation of glutamate to gamma-aminobutyrate. In Escherichia, coli, expression of glutamate decarboxylase (GadB), a 330 kDa hexamer, is, induced to maintain the physiological pH under acidic conditions, like, those of the passage through the stomach en route to the intestine. GadB, together with the antiporter GadC, constitutes the gad acid resistance, system, which confers the ability for bacterial survival for at least 2 h, in a strongly acidic environment. GadB undergoes a pH-dependent, conformational change and exhibits an activity optimum at low pH. We, determined the crystal structures of GadB at acidic and neutral pH. They, reveal the molecular details of the conformational change and the, structural basis for the acidic pH optimum. We demonstrate that the enzyme, is localized exclusively in the cytoplasm at neutral pH, but is recruited, to the membrane when the pH falls. We show by structure-based, site-directed mutagenesis that the triple helix bundle formed by the, N-termini of the protein at acidic pH is the major determinant for this, behaviour.
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<StructureSection load='1pmm' size='340' side='right'caption='[[1pmm]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1pmm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PMM FirstGlance]. <br>
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1PMM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with PLP and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutamate_decarboxylase Glutamate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.15 4.1.1.15] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PMM OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pmm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pmm OCA], [https://pdbe.org/1pmm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pmm RCSB], [https://www.ebi.ac.uk/pdbsum/1pmm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pmm ProSAT]</span></td></tr>
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Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase., Capitani G, De Biase D, Aurizi C, Gut H, Bossa F, Grutter MG, EMBO J. 2003 Aug 15;22(16):4027-37. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12912902 12912902]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DCEB_ECOLI DCEB_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pm/1pmm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pmm ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Glutamate decarboxylase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Aurizi C]]
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[[Category: Aurizi, C.]]
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[[Category: Bossa F]]
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[[Category: Biase, D.De.]]
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[[Category: Capitani G]]
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[[Category: Bossa, F.]]
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[[Category: De Biase D]]
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[[Category: Capitani, G.]]
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[[Category: Grutter MG]]
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[[Category: Grutter, M.G.]]
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[[Category: Gut H]]
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[[Category: Gut, H.]]
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[[Category: ACY]]
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[[Category: PLP]]
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[[Category: low-ph form of gadb]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:55:32 2007''
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Current revision

Crystal structure of Escherichia coli GadB (low pH)

PDB ID 1pmm

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