1xnc

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{{Seed}}
 
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[[Image:1xnc.png|left|200px]]
 
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==THERMOSTABILIZATION OF THE BACILLUS CIRCULANS XYLANASE, BY THE INTRODUCTION OF DISULFIDE BONDS==
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The line below this paragraph, containing "STRUCTURE_1xnc", creates the "Structure Box" on the page.
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<StructureSection load='1xnc' size='340' side='right'caption='[[1xnc]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xnc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XNC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xnc OCA], [https://pdbe.org/1xnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xnc RCSB], [https://www.ebi.ac.uk/pdbsum/1xnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xnc ProSAT]</span></td></tr>
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{{STRUCTURE_1xnc| PDB=1xnc | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/XYNA_NIACI XYNA_NIACI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xn/1xnc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xnc ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The thermostability of the 20 396 Da Bacillus circulans xylanase was increased by the introduction of both intra- and intermolecular disulfide bridges by site-directed mutagenesis. Based on the 3-D structure of the enzyme, sites were chosen where favourable geometry for a bridge existed; in one case, to obtain favourable geometry additional mutations around the cysteine sites were designed by computer modelling. The disulfide bonds introduced into the xylanase were mostly buried and, in the absence of protein denaturants, relatively insensitive to reduction by dithiothreitol. The mutant proteins were examined for residual enzymatic activity after various thermal treatments, and were assayed for enzymatic activity at elevated temperatures to assess their productivity. We have examined one of these mutants by X-ray crystallography. All of the disulfide bond designs tested increased the thermostability of the B. circulans xylanase, but not all enhanced the activity of the enzyme at elevated temperatures.
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===THERMOSTABILIZATION OF THE BACILLUS CIRCULANS XYLANASE, BY THE INTRODUCTION OF DISULFIDE BONDS===
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Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds.,Wakarchuk WW, Sung WL, Campbell RL, Cunningham A, Watson DC, Yaguchi M Protein Eng. 1994 Nov;7(11):1379-86. PMID:7700870<ref>PMID:7700870</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_7700870}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1xnc" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 7700870 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_7700870}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1XNC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNC OCA].
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[[Category: Niallia circulans]]
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[[Category: Campbell RL]]
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==Reference==
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Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds., Wakarchuk WW, Sung WL, Campbell RL, Cunningham A, Watson DC, Yaguchi M, Protein Eng. 1994 Nov;7(11):1379-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7700870 7700870]
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[[Category: Bacillus circulans]]
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[[Category: Endo-1,4-beta-xylanase]]
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[[Category: Single protein]]
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[[Category: Campbell, R L.]]
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[[Category: Glycosidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:34:13 2008''
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Current revision

THERMOSTABILIZATION OF THE BACILLUS CIRCULANS XYLANASE, BY THE INTRODUCTION OF DISULFIDE BONDS

PDB ID 1xnc

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