2bwe

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{{Seed}}
 
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[[Image:2bwe.png|left|200px]]
 
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==The crystal structure of the complex between the UBA and UBL domains of Dsk2==
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The line below this paragraph, containing "STRUCTURE_2bwe", creates the "Structure Box" on the page.
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<StructureSection load='2bwe' size='340' side='right'caption='[[2bwe]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2bwe]] is a 21 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BWE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BWE FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bwe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bwe OCA], [https://pdbe.org/2bwe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bwe RCSB], [https://www.ebi.ac.uk/pdbsum/2bwe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bwe ProSAT]</span></td></tr>
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{{STRUCTURE_2bwe| PDB=2bwe | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DSK2_YEAST DSK2_YEAST] Involved, with RAD23 in spindle pole body duplication. Involved in the ubiquitin-proteasome proteolytic pathway.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/2bwe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bwe ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The yeast proteins Dsk2 and Rad23 belong to a family of proteins that contain an N-terminal ubiquitin-like domain (UBL) and a C-terminal ubiquitin-associated domain (UBA). Both Dsk2 and Rad23 function as adaptors to target ubiquitin-labelled proteins to the proteasome through recognition of polyubiquitin (four or more K48-linked ubiquitins) by their UBA domains and to the yeast proteasomal subunit Rpn1 by their UBL domains. The crystal structures of the Dsk2 UBL domain, the Dsk2 UBA domain and the Dsk2 UBA-UBL complex are reported. In the crystal, the Dsk2 UBA domains associate through electrostatic interactions to form ninefold helical ribbons that leave the ubiquitin-binding surface exposed. The UBA-UBL complex explains the reduced affinity of the UBA domain for UBL compared with ubiquitin and has implications for the regulation of Dsk2 adaptor function during ubiquitin-mediated proteasomal targeting. A model is discussed in which two or more Dsk2 UBA molecules may selectively bind to K48-linked polyubiquitin.
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===THE CRYSTAL STRUCTURE OF THE COMPLEX BETWEEN THE UBA AND UBL DOMAINS OF DSK2===
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Structures of the Dsk2 UBL and UBA domains and their complex.,Lowe ED, Hasan N, Trempe JF, Fonso L, Noble ME, Endicott JA, Johnson LN, Brown NR Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):177-88. Epub 2006, Jan 18. PMID:16421449<ref>PMID:16421449</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16421449}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2bwe" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16421449 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16421449}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2BWE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BWE OCA].
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==Reference==
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Structures of the Dsk2 UBL and UBA domains and their complex., Lowe ED, Hasan N, Trempe JF, Fonso L, Noble ME, Endicott JA, Johnson LN, Brown NR, Acta Crystallogr D Biol Crystallogr. 2006 Feb;62(Pt 2):177-88. Epub 2006, Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16421449 16421449]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Brown NR]]
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[[Category: Brown, N R.]]
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[[Category: Endicott JA]]
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[[Category: Endicott, J A.]]
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[[Category: Fonso L]]
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[[Category: Fonso, L.]]
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[[Category: Hasan N]]
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[[Category: Hasan, N.]]
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[[Category: Johnson LN]]
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[[Category: Johnson, L N.]]
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[[Category: Lowe ED]]
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[[Category: Lowe, E D.]]
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[[Category: Noble MEM]]
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[[Category: Noble, M E.M.]]
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[[Category: Trempe J-F]]
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[[Category: Trempe, J-F.]]
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[[Category: Protein/protein interaction]]
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[[Category: Signaling protein]]
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[[Category: Ubiquitin]]
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[[Category: Ubiquitin-like protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:35:55 2008''
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Current revision

The crystal structure of the complex between the UBA and UBL domains of Dsk2

PDB ID 2bwe

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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