1wb0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:26, 13 December 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1wb0.png|left|200px]]
 
-
<!--
+
==specificity and affinity of natural product cyclopentapeptide inhibitor Argifin against human chitinase==
-
The line below this paragraph, containing "STRUCTURE_1wb0", creates the "Structure Box" on the page.
+
<StructureSection load='1wb0' size='340' side='right'caption='[[1wb0]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1wb0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WB0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WB0 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IAS:BETA-L-ASPARTIC+ACID'>IAS</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=MEA:N-METHYLPHENYLALANINE'>MEA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=VR0:N~5~-[N-(METHYLCARBAMOYL)CARBAMIMIDOYL]-L-ORNITHINE'>VR0</scene></td></tr>
-
{{STRUCTURE_1wb0| PDB=1wb0 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wb0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wb0 OCA], [https://pdbe.org/1wb0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wb0 RCSB], [https://www.ebi.ac.uk/pdbsum/1wb0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wb0 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CHIT1_HUMAN CHIT1_HUMAN] Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.<ref>PMID:7592832</ref> <ref>PMID:7836450</ref> <ref>PMID:9748235</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wb/1wb0_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wb0 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Family 18 chitinases play key roles in organisms ranging from bacteria to man. There is a need for specific, potent inhibitors to probe the function of these chitinases in different organisms. Such molecules could also provide leads for the development of chemotherapeuticals with fungicidal, insecticidal, or anti-inflammatory potential. Recently, two natural product peptides, argifin and argadin, have been characterized, which structurally mimic chitinase-chitooligosaccharide interactions and inhibit a bacterial chitinase in the nM-mM range. Here, we show that these inhibitors also act on human and Aspergillus fumigatus chitinases. The structures of these enzymes in complex with argifin and argadin, together with mutagenesis, fluorescence, and enzymology, reveal that subtle changes in the binding site dramatically affect affinity and selectivity. The data show that it may be possible to develop specific chitinase inhibitors based on the argifin/argadin scaffolds.
-
===SPECIFICITY AND AFFINITY OF NATURAL PRODUCT CYCLOPENTAPEPTIDE INHIBITOR ARGIFIN AGAINST HUMAN CHITINASE===
+
Specificity and affinity of natural product cyclopentapeptide inhibitors against A. fumigatus, human, and bacterial chitinases.,Rao FV, Houston DR, Boot RG, Aerts JM, Hodkinson M, Adams DJ, Shiomi K, Omura S, van Aalten DM Chem Biol. 2005 Jan;12(1):65-76. PMID:15664516<ref>PMID:15664516</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1wb0" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_15664516}}, adds the Publication Abstract to the page
+
*[[Chitinase 3D structures|Chitinase 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 15664516 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_15664516}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
1WB0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WB0 OCA].
+
-
 
+
-
==Reference==
+
-
Specificity and affinity of natural product cyclopentapeptide inhibitors against A. fumigatus, human, and bacterial chitinases., Rao FV, Houston DR, Boot RG, Aerts JM, Hodkinson M, Adams DJ, Shiomi K, Omura S, van Aalten DM, Chem Biol. 2005 Jan;12(1):65-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15664516 15664516]
+
-
[[Category: Chitinase]]
+
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Aalten, D M.F Van.]]
+
[[Category: Synthetic construct]]
-
[[Category: Adams, D J.]]
+
[[Category: Adams DJ]]
-
[[Category: Aerts, J M.F G.]]
+
[[Category: Aerts JMFG]]
-
[[Category: Boot, R G.]]
+
[[Category: Boot RG]]
-
[[Category: Hodkinson, M.]]
+
[[Category: Hodkinson M]]
-
[[Category: Houston, D R.]]
+
[[Category: Houston DR]]
-
[[Category: Omura, S.]]
+
[[Category: Omura S]]
-
[[Category: Rao, F V.]]
+
[[Category: Rao FV]]
-
[[Category: Shiomi, K.]]
+
[[Category: Shiomi K]]
-
[[Category: Alternative splicing]]
+
[[Category: Van Aalten DMF]]
-
[[Category: Argadin]]
+
-
[[Category: Carbohydrate metabolism]]
+
-
[[Category: Chitin degradation]]
+
-
[[Category: Chitin-binding]]
+
-
[[Category: Chitinase inhibitor]]
+
-
[[Category: Cyclopentapeptide inhibitor]]
+
-
[[Category: Glycosidase]]
+
-
[[Category: Human chitinase]]
+
-
[[Category: Hydrolase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:54:29 2008''
+

Current revision

specificity and affinity of natural product cyclopentapeptide inhibitor Argifin against human chitinase

PDB ID 1wb0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wb0"
Personal tools