2hzs

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{{Seed}}
 
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[[Image:2hzs.png|left|200px]]
 
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==Structure of the Mediator head submodule Med8C/18/20==
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The line below this paragraph, containing "STRUCTURE_2hzs", creates the "Structure Box" on the page.
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<StructureSection load='2hzs' size='340' side='right'caption='[[2hzs]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2hzs]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HZS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hzs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hzs OCA], [https://pdbe.org/2hzs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hzs RCSB], [https://www.ebi.ac.uk/pdbsum/2hzs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hzs ProSAT]</span></td></tr>
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{{STRUCTURE_2hzs| PDB=2hzs | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MED20_YEAST MED20_YEAST] Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins.<ref>PMID:16076843</ref> <ref>PMID:16109375</ref> <ref>PMID:16263706</ref> <ref>PMID:16885025</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hz/2hzs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hzs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Mediator head module stimulates basal RNA polymerase II (Pol II) transcription and enables transcriptional regulation. Here we show that the head subunits Med8, Med18 and Med20 form a subcomplex (Med8/18/20) with two submodules. The highly conserved N-terminal domain of Med8 forms one submodule that binds the TATA box-binding protein (TBP) in vitro and is essential in vivo. The second submodule consists of the C-terminal region of Med8 (Med8C), Med18 and Med20. X-ray analysis of this submodule reveals that Med18 and Med20 form related beta-barrel folds. A conserved putative protein-interaction face on the Med8C/18/20 submodule includes sites altered by srb mutations, which counteract defects resulting from Pol II truncation. Our results and published data support a positive role of the Med8/18/20 subcomplex in initiation-complex formation and suggest that the Mediator head contains a multipartite TBP-binding site that can be modulated by transcriptional activators.
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===Structure of the Mediator head submodule Med8C/18/20===
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Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20.,Lariviere L, Geiger S, Hoeppner S, Rother S, Strasser K, Cramer P Nat Struct Mol Biol. 2006 Oct;13(10):895-901. Epub 2006 Sep 10. PMID:16964259<ref>PMID:16964259</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2hzs" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16964259}}, adds the Publication Abstract to the page
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*[[Mediator|Mediator]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16964259 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16964259}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2HZS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HZS OCA].
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==Reference==
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Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20., Lariviere L, Geiger S, Hoeppner S, Rother S, Strasser K, Cramer P, Nat Struct Mol Biol. 2006 Oct;13(10):895-901. Epub 2006 Sep 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16964259 16964259]
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[[Category: Protein complex]]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Cramer, P.]]
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[[Category: Cramer P]]
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[[Category: Geiger, S.]]
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[[Category: Geiger S]]
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[[Category: Hoeppner, S.]]
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[[Category: Hoeppner S]]
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[[Category: Lariviere, L.]]
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[[Category: Lariviere L]]
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[[Category: Rother, S.]]
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[[Category: Rother S]]
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[[Category: Straesser, K.]]
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[[Category: Straesser K]]
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[[Category: Beta barrel]]
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[[Category: Channel]]
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[[Category: Helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:55:58 2008''
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Current revision

Structure of the Mediator head submodule Med8C/18/20

PDB ID 2hzs

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