This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1poa

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1poa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1poa, resolution 1.5&Aring;" /> '''INTERFACIAL CATALYSIS...)
Current revision (05:57, 17 April 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1poa.gif|left|200px]]<br /><applet load="1poa" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1poa, resolution 1.5&Aring;" />
 
-
'''INTERFACIAL CATALYSIS: THE MECHANISM OF PHOSPHOLIPASE A2'''<br />
 
-
==Overview==
+
==INTERFACIAL CATALYSIS: THE MECHANISM OF PHOSPHOLIPASE A2==
-
A chemical description of the action of phospholipase A2 (PLA2) can now be, inferred with confidence from three high-resolution x-ray crystal, structures. The first is the structure of the PLA2 from the venom of the, Chinese cobra (Naja naja atra) in a complex with a phosphonate, transition-state analogue. This enzyme is typical of a large, well-studied, homologous family of PLA2S. The second is a similar complex with the, evolutionarily distant bee-venom PLA2. The third structure is the, uninhibited PLA2 from Chinese cobra venom. Despite the different molecular, architectures of the cobra and bee-venom PLA2s, the transition-state, analogue interacts in a nearly identical way with the catalytic machinery, of both enzymes. The disposition of the fatty-acid side chains suggests a, common access route of the substrate from its position in the lipid, aggregate to its productive interaction with the active site. Comparison, of the cobra-venom complex with the uninhibited enzyme indicates that, optimal binding and catalysis at the lipid-water interface is due to, facilitated substrate diffusion from the interfacial binding surface to, the catalytic site rather than an allosteric change in the enzyme's, structure. However, a second bound calcium ion changes its position upon, the binding of the transition-state analogue, suggesting a mechanism for, augmenting the critical electrophile.
+
<StructureSection load='1poa' size='340' side='right'caption='[[1poa]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1poa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1POA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1POA FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1poa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1poa OCA], [https://pdbe.org/1poa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1poa RCSB], [https://www.ebi.ac.uk/pdbsum/1poa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1poa ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PA2A1_NAJAT PA2A1_NAJAT] Snake venom phospholipase A2 (PLA2) that has high affinity for muscarinic acetylcholine receptors mAChRs (CHRM) and has the ability to activate them. In guinea-pig ileum, produces an onset and dose-dependent contraction. Has also weak anticoagulant activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.<ref>PMID:18281071</ref> <ref>PMID:3117784</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/po/1poa_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1poa ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1POA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1POA OCA].
+
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
Interfacial catalysis: the mechanism of phospholipase A2., Scott DL, White SP, Otwinowski Z, Yuan W, Gelb MH, Sigler PB, Science. 1990 Dec 14;250(4987):1541-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2274785 2274785]
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Naja atra]]
[[Category: Naja atra]]
-
[[Category: Phospholipase A(2)]]
+
[[Category: Otwinowski Z]]
-
[[Category: Single protein]]
+
[[Category: Scott DL]]
-
[[Category: Otwinowski, Z.]]
+
[[Category: Sigler PB]]
-
[[Category: Scott, D.L.]]
+
-
[[Category: Sigler, P.B.]]
+
-
[[Category: CA]]
+
-
[[Category: hydrolase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:58:07 2007''
+

Current revision

INTERFACIAL CATALYSIS: THE MECHANISM OF PHOSPHOLIPASE A2

PDB ID 1poa

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1poa"
Personal tools