This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1pop

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1pop" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pop, resolution 2.1&Aring;" /> '''X-RAY CRYSTALLOGRAPHI...)
Current revision (05:57, 17 April 2024) (edit) (undo)
 
(21 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1pop.gif|left|200px]]<br /><applet load="1pop" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1pop, resolution 2.1&Aring;" />
 
-
'''X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX'''<br />
 
-
==Overview==
+
==X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX==
-
The three-dimensional structure of the papain-leupeptin complex has been, determined by X-ray crystallography to a resolution of 2.1 A (overall, R-factor = 19.8%). The structure indicates that: (i) leupeptin contacts, the S subsites of the papain active site and not the S' subsites; (ii) the, 'carbonyl' carbon atom of the inhibitor is covalently bound by the Cys-25, sulphur atom of papain and is tetrahedrally coordinated; (iii) the, 'carbonyl' oxygen atom of the inhibitor faces the oxyanion hole and makes, hydrogen bond contacts with Gln-19 and Cys-25.
+
<StructureSection load='1pop' size='340' side='right'caption='[[1pop]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1pop]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Carica_papaya Carica papaya] and [https://en.wikipedia.org/wiki/Streptomyces_roseus Streptomyces roseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1POP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1POP FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AR7:AMINO{[(4S)-4-AMINO-5,5-DIHYDROXYPENTYL]AMINO}METHANIMINIUM'>AR7</scene>, <scene name='pdbligand=MOH:METHANOL'>MOH</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pop OCA], [https://pdbe.org/1pop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pop RCSB], [https://www.ebi.ac.uk/pdbsum/1pop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pop ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PAPA1_CARPA PAPA1_CARPA]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/po/1pop_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pop ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1POP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ACE and MOH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1POP OCA].
+
*[[Papain|Papain]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
X-ray crystallographic structure of a papain-leupeptin complex., Schroder E, Phillips C, Garman E, Harlos K, Crawford C, FEBS Lett. 1993 Jan 2;315(1):38-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8416808 8416808]
+
[[Category: Carica papaya]]
-
[[Category: Papain]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Streptomyces roseus]]
-
[[Category: Crawford, C.]]
+
[[Category: Crawford C]]
-
[[Category: Schroder, E.]]
+
[[Category: Schroder E]]
-
[[Category: ACE]]
+
-
[[Category: MOH]]
+
-
[[Category: hydrolase(thiol protease)]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:58:45 2007''
+

Current revision

X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX

PDB ID 1pop

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1pop"
Personal tools