1r74

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{{Seed}}
 
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[[Image:1r74.png|left|200px]]
 
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==Crystal Structure of Human Glycine N-Methyltransferase==
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The line below this paragraph, containing "STRUCTURE_1r74", creates the "Structure Box" on the page.
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<StructureSection load='1r74' size='340' side='right'caption='[[1r74]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1r74]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R74 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr>
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{{STRUCTURE_1r74| PDB=1r74 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r74 OCA], [https://pdbe.org/1r74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r74 RCSB], [https://www.ebi.ac.uk/pdbsum/1r74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r74 ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/GNMT_HUMAN GNMT_HUMAN] Defects in GNMT are the cause of glycine N-methyltransferase deficiency (GNMT deficiency) [MIM:[https://omim.org/entry/606664 606664]; also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.
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== Function ==
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[https://www.uniprot.org/uniprot/GNMT_HUMAN GNMT_HUMAN] Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.<ref>PMID:15340920</ref> <ref>PMID:17660255</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r7/1r74_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r74 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Glycine N-methyltransferases (GNMTs) from three mammalian sources were compared with respect to their crystal structures and kinetic parameters. The crystal structure for the rat enzyme was published previously. Human and mouse GNMT were expressed in Escherichia coli in order to determine their crystal structures. Mouse GNMT was crystallized in two crystal forms, a monoclinic form and a tetragonal form. Comparison of the three structures reveals subtle differences, which may relate to the different kinetic properties of the enzymes.The flexible character of several loops surrounding the active site, along with an analysis of the active site boundaries, indicates that the observed conformations of human and mouse GNMTs are more open than that of the rat enzyme. There is an increase in kcat when going from rat to mouse to human, suggesting a correlation with the increased flexibility of some structural elements of the respective enzymes.
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===Crystal Structure of Human Glycine N-Methyltransferase===
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Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes.,Pakhomova S, Luka Z, Grohmann S, Wagner C, Newcomer ME Proteins. 2004 Nov 1;57(2):331-7. PMID:15340920<ref>PMID:15340920</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15340920}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1r74" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15340920 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15340920}}
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__TOC__
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</StructureSection>
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==Disease==
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Known disease associated with this structure: Glycine N-methyltransferase deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=606628 606628]]
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==About this Structure==
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1R74 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R74 OCA].
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==Reference==
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Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes., Pakhomova S, Luka Z, Grohmann S, Wagner C, Newcomer ME, Proteins. 2004 Nov 1;57(2):331-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15340920 15340920]
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[[Category: Glycine N-methyltransferase]]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Luka, Z.]]
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[[Category: Luka Z]]
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[[Category: Newcomer, M E.]]
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[[Category: Newcomer ME]]
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[[Category: Pakhomova, S.]]
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[[Category: Pakhomova S]]
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[[Category: Wagner, C.]]
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[[Category: Wagner C]]
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[[Category: Glycine n-methyltransferase]]
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[[Category: Human]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:06:34 2008''
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Current revision

Crystal Structure of Human Glycine N-Methyltransferase

PDB ID 1r74

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