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1ppe

From Proteopedia

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(New page: 200px<br /><applet load="1ppe" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ppe, resolution 2.0&Aring;" /> '''THE REFINED 2.0 ANGST...)
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[[Image:1ppe.gif|left|200px]]<br /><applet load="1ppe" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ppe, resolution 2.0&Aring;" />
 
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'''THE REFINED 2.0 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN BOVINE BETA-TRYPSIN AND CMTI-I, A TRYPSIN INHIBITOR FROM SQUASH SEEDS (CUCURBITA MAXIMA): TOPOLOGICAL SIMILARITY OF THE SQUASH SEED INHIBITORS WITH THE CARBOXYPEPTIDASE A INHIBITOR FROM POTATOES'''<br />
 
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==Overview==
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==THE REFINED 2.0 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN BOVINE BETA-TRYPSIN AND CMTI-I, A TRYPSIN INHIBITOR FROM SQUASH SEEDS (CUCURBITA MAXIMA): TOPOLOGICAL SIMILARITY OF THE SQUASH SEED INHIBITORS WITH THE CARBOXYPEPTIDASE A INHIBITOR FROM POTATOES==
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The stoichiometric complex formed between bovine beta-trypsin and the, Cucurbita maxima trypsin inhibitor I (CMTI-I) was crystallized and its, X-ray crystal structure determined using Patterson search techniques. Its, structure has been crystallographically refined to a final R value of, 0.152 (6.0-2.0 A). CMTI-I is of ellipsoidal shape; it lacks helices or, beta-sheets, but consists of turns and connecting short polypeptide, stretches. The disulfide pairing is CYS-3I-20I, Cys-10I-22I and, Cys-16I-28I. According to the polypeptide fold and disulfide connectivity, its structure resembles that of the carboxypeptidase A inhibitor from, potatoes. Thirteen of the 29 inhibitor residues are in direct contact with, trypsin; most of them are in the primary binding segment Val-2I, (P4)-Glu-9I (P4') which contains the reactive site bond Arg-5I-Ile-6I and, is in a conformation observed also for other serine proteinase inhibitors.
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<StructureSection load='1ppe' size='340' side='right'caption='[[1ppe]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ppe]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Cucurbita_maxima Cucurbita maxima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PPE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PPE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ppe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ppe OCA], [https://pdbe.org/1ppe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ppe RCSB], [https://www.ebi.ac.uk/pdbsum/1ppe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ppe ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pp/1ppe_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ppe ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PPE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PPE OCA].
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*[[Trypsin 3D structures|Trypsin 3D structures]]
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*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
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==Reference==
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__TOC__
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The refined 2.0 A X-ray crystal structure of the complex formed between bovine beta-trypsin and CMTI-I, a trypsin inhibitor from squash seeds (Cucurbita maxima). Topological similarity of the squash seed inhibitors with the carboxypeptidase A inhibitor from potatoes., Bode W, Greyling HJ, Huber R, Otlewski J, Wilusz T, FEBS Lett. 1989 Jan 2;242(2):285-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2914611 2914611]
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Protein complex]]
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[[Category: Cucurbita maxima]]
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[[Category: Trypsin]]
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[[Category: Large Structures]]
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[[Category: Bode, W.]]
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[[Category: Bode W]]
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[[Category: Huber, R.]]
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[[Category: Huber R]]
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[[Category: hydrolase(serine proteinase)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 23:59:47 2007''
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Current revision

THE REFINED 2.0 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN BOVINE BETA-TRYPSIN AND CMTI-I, A TRYPSIN INHIBITOR FROM SQUASH SEEDS (CUCURBITA MAXIMA): TOPOLOGICAL SIMILARITY OF THE SQUASH SEED INHIBITORS WITH THE CARBOXYPEPTIDASE A INHIBITOR FROM POTATOES

PDB ID 1ppe

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