1osz

From Proteopedia

(Difference between revisions)

Current revision

MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN

Structural highlights

1osz is a 3 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

B2MG_MOUSE Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The T lineage repertoire is shaped by T cell receptor (TCR)-dependent positive and negative thymic selection processes. Using TCR-transgenic (N15tg) beta2-microglobulin-deficient (beta2m-/-) RAG-2(-/-) H-2(b) mice specific for the VSV8 (RGYVYQGL) octapeptide bound to Kb, we identified a single weak agonist peptide variant V4L (L4) inducing phenotypic and functional T cell maturation. The cognate VSV8 peptide, in contrast, triggers negative selection. The crystal structure of L4/Kb was determined and refined to 2.1 A for comparison with the VSV8/Kb structure at similar resolution. Aside from changes on the p4 side chain of L4 and the resulting alteration of the exposed Kb Lys-66 side chain, these two structures are essentially identical. Hence, a given TCR recognizes subtle distinctions between highly related ligands, resulting in dramatically different selection outcomes. Based on these finding and the recent structural elucidation of the N15-VSV8/Kb complex, moreover, it appears that the germ-line Valpha repertoire contributes in a significant way to positive selection.

Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands.,Ghendler Y, Teng MK, Liu JH, Witte T, Liu J, Kim KS, Kern P, Chang HC, Wang JH, Reinherz EL Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10061-6. PMID:9707600^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ghendler Y, Teng MK, Liu JH, Witte T, Liu J, Kim KS, Kern P, Chang HC, Wang JH, Reinherz EL. Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands. Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10061-6. PMID:9707600

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1osz"

@@ Line 1: / Line 1: @@
-{{Seed}}
-[[Image:1osz.png|left|200px]]
-<!--
+==MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN==
-The line below this paragraph, containing "STRUCTURE_1osz", creates the "Structure Box" on the page.
+<StructureSection load='1osz' size='340' side='right'caption='[[1osz]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[1osz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OSZ FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1osz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1osz OCA], [https://pdbe.org/1osz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1osz RCSB], [https://www.ebi.ac.uk/pdbsum/1osz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1osz ProSAT]</span></td></tr>
-{{STRUCTURE_1osz|  PDB=1osz  |  SCENE=  }}
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/B2MG_MOUSE B2MG_MOUSE] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/os/1osz_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1osz ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The T lineage repertoire is shaped by T cell receptor (TCR)-dependent positive and negative thymic selection processes. Using TCR-transgenic (N15tg) beta2-microglobulin-deficient (beta2m-/-) RAG-2(-/-) H-2(b) mice specific for the VSV8 (RGYVYQGL) octapeptide bound to Kb, we identified a single weak agonist peptide variant V4L (L4) inducing phenotypic and functional T cell maturation. The cognate VSV8 peptide, in contrast, triggers negative selection. The crystal structure of L4/Kb was determined and refined to 2.1 A for comparison with the VSV8/Kb structure at similar resolution. Aside from changes on the p4 side chain of L4 and the resulting alteration of the exposed Kb Lys-66 side chain, these two structures are essentially identical. Hence, a given TCR recognizes subtle distinctions between highly related ligands, resulting in dramatically different selection outcomes. Based on these finding and the recent structural elucidation of the N15-VSV8/Kb complex, moreover, it appears that the germ-line Valpha repertoire contributes in a significant way to positive selection.
-===MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN===
+Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands.,Ghendler Y, Teng MK, Liu JH, Witte T, Liu J, Kim KS, Kern P, Chang HC, Wang JH, Reinherz EL Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10061-6. PMID:9707600<ref>PMID:9707600</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 1osz" style="background-color:#fffaf0;"></div>
-<!--
+==See Also==
-The line below this paragraph, {{ABSTRACT_PUBMED_9707600}}, adds the Publication Abstract to the page
+*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
-(as it appears on PubMed at http://www.pubmed.gov), where 9707600 is the PubMed ID number.
+== References ==
--->
+<references/>
-{{ABSTRACT_PUBMED_9707600}}
+__TOC__
+</StructureSection>
-==About this Structure==
+[[Category: Large Structures]]
-OSZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OSZ OCA].
-==Reference==
-Differential thymic selection outcomes stimulated by focal structural alteration in peptide/major histocompatibility complex ligands., Ghendler Y, Teng MK, Liu JH, Witte T, Liu J, Kim KS, Kern P, Chang HC, Wang JH, Reinherz EL, Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):10061-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9707600 9707600]
 [[Category: Mus musculus]]
-[[Category: Protein complex]]
+[[Category: Chang H-C]]
-[[Category: Chang, H C.]]
+[[Category: Ghendler Y]]
-[[Category: Ghendler, Y.]]
+[[Category: Kern P]]
-[[Category: Kern, P.]]
+[[Category: Kim KS]]
-[[Category: Kim, K S.]]
+[[Category: Liu J]]
-[[Category: Liu, J.]]
+[[Category: Liu J-H]]
-[[Category: Liu, J H.]]
+[[Category: Reinherz EL]]
-[[Category: Reinherz, E L.]]
+[[Category: Teng M-K]]
-[[Category: Teng, M K.]]
+[[Category: Wang J-H]]
-[[Category: Wang, J H.]]
+[[Category: Witte T]]
-[[Category: Witte, T.]]
-[[Category: Mhc/peptide complex]]
-[[Category: Thymic selection]]
-[[Category: Transmembrane protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:48:06 2008''

1osz

From Proteopedia

Current revision

MHC CLASS I H-2KB HEAVY CHAIN COMPLEXED WITH BETA-2 MICROGLOBULIN AND AN (L4V) MUTANT OF THE VESICULAR STOMATITIS VIRUS NUCLEOPROTEIN

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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