1psh

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(New page: 200px<br /><applet load="1psh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1psh, resolution 2.3&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1psh.gif|left|200px]]<br /><applet load="1psh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1psh, resolution 2.3&Aring;" />
 
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'''CRYSTAL STRUCTURE OF PHOSPHOLIPASE A2 FROM INDIAN COBRA REVEALS A TRIMERIC ASSOCIATION'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF PHOSPHOLIPASE A2 FROM INDIAN COBRA REVEALS A TRIMERIC ASSOCIATION==
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Phospholipase A2 (PLA2) from Indian cobra venom (Naja naja naja) was, crystallized from ethanol in space group P4(3)2(1)2 in the presence of, Ca2+. The x-ray crystal structure was determined to 2.3-A resolution by, molecular replacement techniques using a theoretical model constructed, from homologous segments of the bovine pancreatic, porcine pancreatic, and, rattlesnake venom crystal structures. The structure was refined to an R, value of 0.174 for 17,542 reflections between 6.0- and 2.3-A resolution (F, &gt; 2 sigma), including 148 water molecules. The 119-amino acid enzyme has, an overall architecture strikingly similar to the other known PLA2, structures with regions implicated in catalysis showing the greatest, structural conservation. Unexpectedly, three monomers were found to occupy, the asymmetric unit and are oriented with their catalytic sites facing the, pseudo-threefold axis with approximately 15% of the solvent accessible, surface of each monomer buried in trimer contacts. The majority of the, interactions at the subunit interfaces are made by residues unique to PLA2, sequences from cobra and krait venoms. The possible relevance of this, unique trimeric structure is considered.
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<StructureSection load='1psh' size='340' side='right'caption='[[1psh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1psh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_naja Naja naja]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PSH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1psh OCA], [https://pdbe.org/1psh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1psh RCSB], [https://www.ebi.ac.uk/pdbsum/1psh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1psh ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA2A2_NAJNA PA2A2_NAJNA] PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/1psh_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1psh ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Phospholipase A2 (PLA2) from Indian cobra venom (Naja naja naja) was crystallized from ethanol in space group P4(3)2(1)2 in the presence of Ca2+. The x-ray crystal structure was determined to 2.3-A resolution by molecular replacement techniques using a theoretical model constructed from homologous segments of the bovine pancreatic, porcine pancreatic, and rattlesnake venom crystal structures. The structure was refined to an R value of 0.174 for 17,542 reflections between 6.0- and 2.3-A resolution (F &gt; 2 sigma), including 148 water molecules. The 119-amino acid enzyme has an overall architecture strikingly similar to the other known PLA2 structures with regions implicated in catalysis showing the greatest structural conservation. Unexpectedly, three monomers were found to occupy the asymmetric unit and are oriented with their catalytic sites facing the pseudo-threefold axis with approximately 15% of the solvent accessible surface of each monomer buried in trimer contacts. The majority of the interactions at the subunit interfaces are made by residues unique to PLA2 sequences from cobra and krait venoms. The possible relevance of this unique trimeric structure is considered.
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==About this Structure==
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Crystal structure of phospholipase A2 from Indian cobra reveals a trimeric association.,Fremont DH, Anderson DH, Wilson IA, Dennis EA, Xuong NH Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):342-6. PMID:8419939<ref>PMID:8419939</ref>
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1PSH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Naja_naja Naja naja] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PSH OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of phospholipase A2 from Indian cobra reveals a trimeric association., Fremont DH, Anderson DH, Wilson IA, Dennis EA, Xuong NH, Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):342-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8419939 8419939]
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</div>
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[[Category: Naja naja]]
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<div class="pdbe-citations 1psh" style="background-color:#fffaf0;"></div>
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[[Category: Phospholipase A(2)]]
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[[Category: Single protein]]
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[[Category: Fremont, D.]]
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[[Category: Wilson, I.]]
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[[Category: Xuong, N.H.]]
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[[Category: CA]]
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[[Category: carboxylic ester hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:05:14 2007''
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==See Also==
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Naja naja]]
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[[Category: Fremont D]]
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[[Category: Wilson I]]
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[[Category: Xuong N-H]]

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CRYSTAL STRUCTURE OF PHOSPHOLIPASE A2 FROM INDIAN COBRA REVEALS A TRIMERIC ASSOCIATION

PDB ID 1psh

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