1psu

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(New page: 200px<br /><applet load="1psu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1psu, resolution 2.20&Aring;" /> '''Structure of the E. ...)
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[[Image:1psu.gif|left|200px]]<br /><applet load="1psu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1psu, resolution 2.20&Aring;" />
 
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'''Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon'''<br />
 
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==About this Structure==
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==Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon==
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1PSU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PSU OCA].
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<StructureSection load='1psu' size='340' side='right'caption='[[1psu]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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[[Category: Escherichia coli]]
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== Structural highlights ==
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[[Category: Single protein]]
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<table><tr><td colspan='2'>[[1psu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PSU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PSU FirstGlance]. <br>
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[[Category: Buglino, J.]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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[[Category: Burley, S.K.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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[[Category: Kniewel, R.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1psu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1psu OCA], [https://pdbe.org/1psu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1psu RCSB], [https://www.ebi.ac.uk/pdbsum/1psu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1psu ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1psu TOPSAN]</span></td></tr>
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[[Category: Lima, C.D.]]
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</table>
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[[Category: NYSGXRC, New.York.Structural.GenomiX.Research.Consortium.]]
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== Function ==
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[[Category: Solorzano, V.]]
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[https://www.uniprot.org/uniprot/PAAI_ECOLI PAAI_ECOLI] Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA.<ref>PMID:9748275</ref> <ref>PMID:16464851</ref>
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[[Category: Wu, J.]]
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== Evolutionary Conservation ==
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[[Category: degradation]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: new york structural genomix research consortium]]
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Check<jmol>
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[[Category: nysgxrc]]
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<jmolCheckbox>
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[[Category: operon]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/1psu_consurf.spt"</scriptWhenChecked>
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[[Category: phenylacetic acid]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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[[Category: protein structure initiative]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: psi]]
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</jmolCheckbox>
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[[Category: structural genomics]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1psu ConSurf].
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[[Category: t820]]
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure and biochemical function of the hot dog-fold thioesterase PaaI operative in the aerobic phenylacetate degradation pathway are examined. PaaI showed modest activity with phenylacetyl-coenzyme A, suggestive of a role in coenzyme A release from this pathway intermediate in the event of limiting downstream pathway enzymes. Minimal activity was observed with aliphatic acyl-coenzyme A thioesters, which ruled out PaaI function in the lower phenylacetate pathway. PaaI was most active with ring-hydroxylated phenylacetyl-coenzyme A thioesters. The x-ray crystal structure of the Escherichia coli thioesterase is reported and analyzed to define the structural basis of substrate recognition and catalysis. The contributions of catalytic and substrate binding residues, thus, identified were examined through steady-state kinetic analysis of site-directed mutant proteins.
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:05:40 2007''
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Structure, function, and mechanism of the phenylacetate pathway hot dog-fold thioesterase PaaI.,Song F, Zhuang Z, Finci L, Dunaway-Mariano D, Kniewel R, Buglino JA, Solorzano V, Wu J, Lima CD J Biol Chem. 2006 Apr 21;281(16):11028-38. Epub 2006 Feb 7. PMID:16464851<ref>PMID:16464851</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1psu" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Buglino J]]
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[[Category: Burley SK]]
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[[Category: Kniewel R]]
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[[Category: Lima CD]]
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[[Category: Solorzano V]]
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[[Category: Wu J]]

Current revision

Structure of the E. coli PaaI protein from the phyenylacetic acid degradation operon

PDB ID 1psu

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