1psz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1psz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1psz, resolution 2.0&Aring;" /> '''PNEUMOCOCCAL SURFACE ...)
Current revision (23:47, 27 December 2023) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1psz.gif|left|200px]]<br /><applet load="1psz" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1psz, resolution 2.0&Aring;" />
 
-
'''PNEUMOCOCCAL SURFACE ANTIGEN PSAA'''<br />
 
-
==Overview==
+
==PNEUMOCOCCAL SURFACE ANTIGEN PSAA==
-
Background:. The surface protein PsaA of the pathogenic bacterium, Streptococcus pneumoniae plays an essential role in its virulence. PsaA is, a putative ATP-binding cassette-type (ABC-type) binding protein involved, in the uptake of Mn2+ and possibly Zn2+ and is considered to be both a, potential drug target and and a candidate vaccine component. Results:. The, structure of PsaA has been determined to 2.0 A resolution using X-ray, crystallography and is the first structure obtained for an ABC-type, binding protein from a Gram-positive organism. The protein consists of two, (beta/alpha)4 domains linked together by a single helix. A metal-binding, site is formed in the domain interface by the sidechains of His67, His139, Glu205 and Asp280 and is occupied in the structure. Conclusions:. The, structural topology of PsaA is fundamentally different from that of other, ABC-type binding proteins determined thus far in that PsaA lacks the, characteristic 'hinge peptides' involved in conformational change upon, solute uptake and release. In our structure, the metal-binding site is, probably occupied by Zn2+. The site seems to be well conserved amongst, related receptors from both Gram-positive and Gram-negative bacteria.
+
<StructureSection load='1psz' size='340' side='right'caption='[[1psz]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1psz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PSZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PSZ FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1psz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1psz OCA], [https://pdbe.org/1psz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1psz RCSB], [https://www.ebi.ac.uk/pdbsum/1psz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1psz ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MTSA_STRPN MTSA_STRPN] Part of an ATP-driven transport system for manganese. Also act as an adhesin which is involved on adherence to extracellular matrix. It is an important factor in pathogenesis and infection.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ps/1psz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1psz ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Background:. The surface protein PsaA of the pathogenic bacterium Streptococcus pneumoniae plays an essential role in its virulence. PsaA is a putative ATP-binding cassette-type (ABC-type) binding protein involved in the uptake of Mn2+ and possibly Zn2+ and is considered to be both a potential drug target and and a candidate vaccine component. Results:. The structure of PsaA has been determined to 2.0 A resolution using X-ray crystallography and is the first structure obtained for an ABC-type binding protein from a Gram-positive organism. The protein consists of two (beta/alpha)4 domains linked together by a single helix. A metal-binding site is formed in the domain interface by the sidechains of His67, His139, Glu205 and Asp280 and is occupied in the structure. Conclusions:. The structural topology of PsaA is fundamentally different from that of other ABC-type binding proteins determined thus far in that PsaA lacks the characteristic 'hinge peptides' involved in conformational change upon solute uptake and release. In our structure, the metal-binding site is probably occupied by Zn2+. The site seems to be well conserved amongst related receptors from both Gram-positive and Gram-negative bacteria.
-
==About this Structure==
+
The crystal structure of pneumococcal surface antigen PsaA reveals a metal-binding site and a novel structure for a putative ABC-type binding protein.,Lawrence MC, Pilling PA, Epa VC, Berry AM, Ogunniyi AD, Paton JC Structure. 1998 Dec 15;6(12):1553-61. PMID:9862808<ref>PMID:9862808</ref>
-
1PSZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PSZ OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
The crystal structure of pneumococcal surface antigen PsaA reveals a metal-binding site and a novel structure for a putative ABC-type binding protein., Lawrence MC, Pilling PA, Epa VC, Berry AM, Ogunniyi AD, Paton JC, Structure. 1998 Dec 15;6(12):1553-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9862808 9862808]
+
</div>
-
[[Category: Single protein]]
+
<div class="pdbe-citations 1psz" style="background-color:#fffaf0;"></div>
-
[[Category: Streptococcus pneumoniae]]
+
-
[[Category: Berry, A.M.]]
+
-
[[Category: Epa, V.C.]]
+
-
[[Category: Lawrence, M.C.]]
+
-
[[Category: Ogunniyi, A.D.]]
+
-
[[Category: Paton, J.C.]]
+
-
[[Category: Pilling, P.A.]]
+
-
[[Category: ZN]]
+
-
[[Category: abc-type binding protein]]
+
-
[[Category: metal-binding protein]]
+
-
[[Category: pneumococcal surface antigen]]
+
-
[[Category: psaa]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:05:50 2007''
+
==See Also==
 +
*[[Adhesin 3D structures|Adhesin 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Streptococcus pneumoniae]]
 +
[[Category: Berry AM]]
 +
[[Category: Epa VC]]
 +
[[Category: Lawrence MC]]
 +
[[Category: Ogunniyi AD]]
 +
[[Category: Paton JC]]
 +
[[Category: Pilling PA]]

Current revision

PNEUMOCOCCAL SURFACE ANTIGEN PSAA

PDB ID 1psz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1psz"
Personal tools