1s36

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{{Seed}}
 
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[[Image:1s36.png|left|200px]]
 
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==Crystal structure of a Ca2+-discharged photoprotein: Implications for the mechanisms of the calcium trigger and the bioluminescence==
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The line below this paragraph, containing "STRUCTURE_1s36", creates the "Structure Box" on the page.
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<StructureSection load='1s36' size='340' side='right'caption='[[1s36]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1s36]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S36 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S36 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.96&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CEI:N-[3-BENZYL-5-(4-HYDROXYPHENYL)PYRAZIN-2-YL]-2-(4-HYDROXYPHENYL)ACETAMIDE'>CEI</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_1s36| PDB=1s36 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s36 OCA], [https://pdbe.org/1s36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s36 RCSB], [https://www.ebi.ac.uk/pdbsum/1s36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s36 ProSAT], [https://www.topsan.org/Proteins/SECSG/1s36 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OBL_OBELO OBL_OBELO] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s3/1s36_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s36 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ca2+-regulated photoproteins are members of the EF-hand calcium-binding protein family. The addition of Ca2+ produces a blue bioluminescence by triggering a decarboxylation reaction of protein-bound hydroperoxycoelenterazine to form the product, coelenteramide, in an excited state. Based on the spatial structures of aequorin and several obelins, we have postulated mechanisms for the Ca2+ trigger and for generation of the different excited states that are the origin of the different colors of bioluminescence. Here we report the crystal structure of the Ca2+-discharged photoprotein obelin at 1.96-A resolution. The results lend support to the proposed mechanisms and provide new structural insight into details of these processes. Global conformational changes caused by Ca2+ association are typical of the class of calcium signal modulators within the EF-hand protein superfamily. Accommodation of the Ca2+ ions into the loops of the EF-hands is seen to propagate into the active site of the protein now occupied by the coelenteramide where there is a significant repositioning and flipping of the His-175 imidazole ring as crucially required in the trigger hypothesis. Also the H-bonding between His-22 and the coelenterazine found in the active photoprotein is preserved at the equivalent position of coelenteramide, confirming the proposed rapid excited state proton transfer that would lead to the excited state of the phenolate ion pair, which is responsible for the blue emission of bioluminescence.
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===Crystal structure of a Ca2+-discharged photoprotein: Implications for the mechanisms of the calcium trigger and the bioluminescence===
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Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence.,Deng L, Markova SV, Vysotski ES, Liu ZJ, Lee J, Rose J, Wang BC J Biol Chem. 2004 Aug 6;279(32):33647-52. Epub 2004 May 20. PMID:15155735<ref>PMID:15155735</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15155735}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1s36" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15155735 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15155735}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1S36 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S36 OCA].
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==Reference==
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Crystal structure of a Ca2+-discharged photoprotein: implications for mechanisms of the calcium trigger and bioluminescence., Deng L, Markova SV, Vysotski ES, Liu ZJ, Lee J, Rose J, Wang BC, J Biol Chem. 2004 Aug 6;279(32):33647-52. Epub 2004 May 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15155735 15155735]
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[[Category: Obelia longissima]]
[[Category: Obelia longissima]]
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[[Category: Single protein]]
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[[Category: Deng L]]
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[[Category: Deng, L.]]
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[[Category: Lee J]]
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[[Category: Lee, J.]]
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[[Category: Liu Z-J]]
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[[Category: Liu, Z J.]]
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[[Category: Markova SV]]
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[[Category: Markova, S V.]]
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[[Category: Rose J]]
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[[Category: Rose, J.]]
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[[Category: Vysotski ES]]
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[[Category: SECSG, Southeast Collaboratory for Structural Genomics.]]
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[[Category: Wang B-C]]
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[[Category: Vysotski, E S.]]
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[[Category: Wang, B C.]]
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[[Category: Aequorin]]
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[[Category: Bioluminescence]]
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[[Category: Calcium binding]]
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[[Category: Ef-hand]]
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[[Category: Obelin]]
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[[Category: Photoprotein]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Secsg]]
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[[Category: Southeast collaboratory for structural genomic]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:42:29 2008''
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Current revision

Crystal structure of a Ca2+-discharged photoprotein: Implications for the mechanisms of the calcium trigger and the bioluminescence

PDB ID 1s36

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