1pv6

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(New page: 200px<br /><applet load="1pv6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pv6, resolution 3.5&Aring;" /> '''Crystal structure of ...)
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[[Image:1pv6.gif|left|200px]]<br /><applet load="1pv6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pv6, resolution 3.5&Aring;" />
 
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'''Crystal structure of lactose permease'''<br />
 
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==Overview==
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==Crystal structure of lactose permease==
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Membrane transport proteins that transduce free energy stored in, electrochemical ion gradients into a concentration gradient are a major, class of membrane proteins. We report the crystal structure at 3.5, angstroms of the Escherichia coli lactose permease, an intensively studied, member of the major facilitator superfamily of transporters. The molecule, is composed of N- and C-terminal domains, each with six transmembrane, helices, symmetrically positioned within the permease. A large internal, hydrophilic cavity open to the cytoplasmic side represents the, inward-facing conformation of the transporter. The structure with a bound, lactose homolog, beta-D-galactopyranosyl-1-thio-beta-D-galactopyranoside, reveals the sugar-binding site in the cavity, and residues that play major, roles in substrate recognition and proton translocation are identified. We, propose a possible mechanism for lactose/proton symport (co-transport), consistent with both the structure and a large body of experimental data.
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<StructureSection load='1pv6' size='340' side='right'caption='[[1pv6]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pv6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PV6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pv6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pv6 OCA], [https://pdbe.org/1pv6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pv6 RCSB], [https://www.ebi.ac.uk/pdbsum/1pv6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pv6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACY_ECOLI LACY_ECOLI] Responsible for transport of beta-galactosides into the cell, with the concomitant import of a proton (symport system).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pv/1pv6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pv6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Membrane transport proteins that transduce free energy stored in electrochemical ion gradients into a concentration gradient are a major class of membrane proteins. We report the crystal structure at 3.5 angstroms of the Escherichia coli lactose permease, an intensively studied member of the major facilitator superfamily of transporters. The molecule is composed of N- and C-terminal domains, each with six transmembrane helices, symmetrically positioned within the permease. A large internal hydrophilic cavity open to the cytoplasmic side represents the inward-facing conformation of the transporter. The structure with a bound lactose homolog, beta-D-galactopyranosyl-1-thio-beta-D-galactopyranoside, reveals the sugar-binding site in the cavity, and residues that play major roles in substrate recognition and proton translocation are identified. We propose a possible mechanism for lactose/proton symport (co-transport) consistent with both the structure and a large body of experimental data.
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==About this Structure==
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Structure and mechanism of the lactose permease of Escherichia coli.,Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S Science. 2003 Aug 1;301(5633):610-5. PMID:12893935<ref>PMID:12893935</ref>
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1PV6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1PV6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure and mechanism of the lactose permease of Escherichia coli., Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S, Science. 2003 Aug 1;301(5633):610-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12893935 12893935]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1pv6" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Abramson, J.]]
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[[Category: Iwata, S.]]
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[[Category: Kaback, H.R.]]
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[[Category: Kasho, V.]]
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[[Category: Smirnova, I.]]
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[[Category: Verner, G.]]
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[[Category: membrane protein]]
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[[Category: sugar transport]]
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[[Category: symport]]
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[[Category: transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:08:52 2007''
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==See Also==
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*[[Lactose Permease|Lactose Permease]]
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*[[Symporter 3D structures|Symporter 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Abramson J]]
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[[Category: Iwata S]]
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[[Category: Kaback HR]]
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[[Category: Kasho V]]
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[[Category: Smirnova I]]
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[[Category: Verner G]]

Current revision

Crystal structure of lactose permease

PDB ID 1pv6

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