2ghj

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{{Seed}}
 
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[[Image:2ghj.png|left|200px]]
 
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==Crystal structure of folded and partially unfolded forms of Aquifex aeolicus ribosomal protein L20==
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The line below this paragraph, containing "STRUCTURE_2ghj", creates the "Structure Box" on the page.
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<StructureSection load='2ghj' size='340' side='right'caption='[[2ghj]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2ghj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GHJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2ghj| PDB=2ghj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ghj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ghj OCA], [https://pdbe.org/2ghj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ghj RCSB], [https://www.ebi.ac.uk/pdbsum/2ghj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ghj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RL20_AQUAE RL20_AQUAE] Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2ghj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ghj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The recent finding of intrinsically unstructured proteins defies the classical structure-function paradigm. However, owing to their flexibility, intrinsically unstructured proteins generally escape detailed structural investigations. Consequently little is known about the extent of conformational disorder and its role in biological functions. Here, we present the X-ray structure of the unbound ribosomal protein L20, the long basic amino-terminal extension of which has been previously interpreted as fully disordered in the absence of RNA. This study provides the first detailed picture of two protein folding states trapped together in a crystal and indicates that unfolding occurs in discrete regions of the whole protein, corresponding mainly to RNA-binding residues. The electrostatic destabilization of the long alpha-helix and a structural communication between the two L20 domains are reminiscent of those observed in calmodulin. The detailed comparison of the two conformations observed in the crystal provides new insights into the role of unfolded extensions in ribosomal assembly.
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===Crystal structure of folded and partially unfolded forms of Aquifex aeolicus ribosomal protein L20===
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Coexistence of two protein folding states in the crystal structure of ribosomal protein L20.,Timsit Y, Allemand F, Chiaruttini C, Springer M EMBO Rep. 2006 Oct;7(10):1013-8. Epub 2006 Sep 15. PMID:16977336<ref>PMID:16977336</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2ghj" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_16977336}}, adds the Publication Abstract to the page
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*[[Ribosomal protein L20|Ribosomal protein L20]]
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(as it appears on PubMed at http://www.pubmed.gov), where 16977336 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16977336}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2GHJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GHJ OCA].
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==Reference==
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Coexistence of two protein folding states in the crystal structure of ribosomal protein L20., Timsit Y, Allemand F, Chiaruttini C, Springer M, EMBO Rep. 2006 Oct;7(10):1013-8. Epub 2006 Sep 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16977336 16977336]
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[[Category: Aquifex aeolicus]]
[[Category: Aquifex aeolicus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Allemand, F.]]
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[[Category: Allemand F]]
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[[Category: Chiaruttini, C.]]
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[[Category: Chiaruttini C]]
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[[Category: Springer, M.]]
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[[Category: Springer M]]
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[[Category: Timsit, Y.]]
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[[Category: Timsit Y]]
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[[Category: Folding intermediate]]
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[[Category: Ribosomal protein extension]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:00:45 2008''
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Current revision

Crystal structure of folded and partially unfolded forms of Aquifex aeolicus ribosomal protein L20

PDB ID 2ghj

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