2gth

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{{Seed}}
 
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[[Image:2gth.png|left|200px]]
 
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==crystal structure of the wildtype MHV coronavirus non-structural protein nsp15==
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The line below this paragraph, containing "STRUCTURE_2gth", creates the "Structure Box" on the page.
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<StructureSection load='2gth' size='340' side='right'caption='[[2gth]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gth]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_A59 Murine hepatitis virus strain A59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GTH FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gth OCA], [https://pdbe.org/2gth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gth RCSB], [https://www.ebi.ac.uk/pdbsum/2gth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gth ProSAT]</span></td></tr>
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{{STRUCTURE_2gth| PDB=2gth | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9J3E8_9BETC Q9J3E8_9BETC] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.[ARBA:ARBA00003368]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/2gth_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gth ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The unique coronavirus transcription/replication machinery comprised of multiple virus-encoded nonstructural proteins (nsp) plays a vital role during initial and intermediate phases of the viral life cycle. The crystal structure of mouse hepatitis virus strain A59 (MHV-A59) nsp15 is reported at 2.15-A resolution. nsp15 is an XendoU endoribonuclease and is the first one from this family to have its structure unveiled. The MHV-A59 nsp15 monomer structure has a novel protein fold. Two nsp15 trimers form a back-to-back hexamer that is believed to be the functional unit. The structure reveals the catalytic site including the highly conserved residues His262, His277, and Lys317, which is supported by mutagenesis analysis. Gel filtration and enzyme activity assays confirmed that the hexamer is the active form for nsp15 and demonstrate the specificity of nsp15 for uridylate. The high sequence conservation of nsp15 in coronaviruses, including that of severe acute respiratory syndrome, suggests that this protein may provide a new target for the design of antiviral therapeutics.
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===crystal structure of the wildtype MHV coronavirus non-structural protein nsp15===
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New antiviral target revealed by the hexameric structure of mouse hepatitis virus nonstructural protein nsp15.,Xu X, Zhai Y, Sun F, Lou Z, Su D, Xu Y, Zhang R, Joachimiak A, Zhang XC, Bartlam M, Rao Z J Virol. 2006 Aug;80(16):7909-17. PMID:16873248<ref>PMID:16873248</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16873248}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2gth" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16873248 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16873248}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2GTH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Murine_hepatitis_virus Murine hepatitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GTH OCA].
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[[Category: Murine hepatitis virus strain A59]]
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[[Category: Lou Z]]
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==Reference==
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[[Category: Rao Z]]
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New antiviral target revealed by the hexameric structure of mouse hepatitis virus nonstructural protein nsp15., Xu X, Zhai Y, Sun F, Lou Z, Su D, Xu Y, Zhang R, Joachimiak A, Zhang XC, Bartlam M, Rao Z, J Virol. 2006 Aug;80(16):7909-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16873248 16873248]
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[[Category: Su D]]
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[[Category: Murine hepatitis virus]]
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[[Category: Sun F]]
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[[Category: Single protein]]
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[[Category: Xu X]]
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[[Category: Lou, Z.]]
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[[Category: Zhai Y]]
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[[Category: Rao, Z.]]
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[[Category: Su, D.]]
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[[Category: Sun, F.]]
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[[Category: Xu, X.]]
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[[Category: Zhai, Y.]]
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[[Category: Mhv]]
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[[Category: Nsp15]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:23:14 2008''
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Current revision

crystal structure of the wildtype MHV coronavirus non-structural protein nsp15

PDB ID 2gth

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