2dpf

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{{Seed}}
 
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[[Image:2dpf.png|left|200px]]
 
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==Crystal Structure of curculin1 homodimer==
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The line below this paragraph, containing "STRUCTURE_2dpf", creates the "Structure Box" on the page.
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<StructureSection load='2dpf' size='340' side='right'caption='[[2dpf]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2dpf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Molineria_latifolia Molineria latifolia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DPF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2dpf| PDB=2dpf | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dpf OCA], [https://pdbe.org/2dpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dpf RCSB], [https://www.ebi.ac.uk/pdbsum/2dpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dpf ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CURC1_MOLLA CURC1_MOLLA] Taste-modifying protein; sweet-tasting. After curculin, water elicits a sweet taste, and sour substances induce a stronger sense of sweetness.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dp/2dpf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dpf ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Curculin isolated from Curculigo latifolia, a plant grown in Malaysia, has an intriguing property of modifying sour taste into sweet taste. In addition to this taste-modifying activity, curculin itself elicits a sweet taste. Although these activities have been attributed to the heterodimeric isoform and not homodimers of curculin, the underlying mechanisms for the dual action of this protein have been largely unknown. To identify critical sites for these activities, we performed a mutational and structural study of recombinant curculin. Based on the comparison of crystal structures of curculin homo- and heterodimers, a series of mutants was designed and subjected to tasting assays. Mapping of amino acid residues on the three-dimensional structure according to their mutational effects revealed that the curculin heterodimer exhibits sweet-tasting and taste-modifying activities through its partially overlapping but distinct molecular surfaces. These findings suggest that the two activities of the curculin heterodimer are expressed through its two different modes of interactions with the T1R2-T1R3 heterodimeric sweet taste receptor.
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===Crystal Structure of curculin1 homodimer===
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Curculin exhibits sweet-tasting and taste-modifying activities through its distinct molecular surfaces.,Kurimoto E, Suzuki M, Amemiya E, Yamaguchi Y, Nirasawa S, Shimba N, Xu N, Kashiwagi T, Kawai M, Suzuki E, Kato K J Biol Chem. 2007 Nov 16;282(46):33252-6. Epub 2007 Sep 25. PMID:17895249<ref>PMID:17895249</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17895249}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2dpf" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17895249 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17895249}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2DPF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Curculigo_latifolia Curculigo latifolia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DPF OCA].
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[[Category: Molineria latifolia]]
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[[Category: Amemiya E]]
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==Reference==
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[[Category: Kashiwagi T]]
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Curculin exhibits sweet-tasting and taste-modifying activities through its distinct molecular surfaces., Kurimoto E, Suzuki M, Amemiya E, Yamaguchi Y, Nirasawa S, Shimba N, Xu N, Kashiwagi T, Kawai M, Suzuki E, Kato K, J Biol Chem. 2007 Nov 16;282(46):33252-6. Epub 2007 Sep 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17895249 17895249]
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[[Category: Kato K]]
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[[Category: Curculigo latifolia]]
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[[Category: Kawai M]]
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[[Category: Single protein]]
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[[Category: Kurimoto E]]
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[[Category: Amemiya, E.]]
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[[Category: Nirasawa S]]
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[[Category: Kashiwagi, T.]]
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[[Category: Shimba N]]
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[[Category: Kato, K.]]
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[[Category: Suzuki E]]
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[[Category: Kawai, M.]]
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[[Category: Suzuki M]]
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[[Category: Kurimoto, E.]]
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[[Category: Xu N]]
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[[Category: Nirasawa, S.]]
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[[Category: Yamaguchi Y]]
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[[Category: Shimba, N.]]
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[[Category: Suzuki, E.]]
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[[Category: Suzuki, M.]]
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[[Category: Xu, N.]]
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[[Category: Yamaguchi, Y.]]
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[[Category: Plant protein]]
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[[Category: Sweet taste]]
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[[Category: Taste modifying]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:38:41 2008''
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Current revision

Crystal Structure of curculin1 homodimer

PDB ID 2dpf

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OCA

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