1r5n

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{{Seed}}
 
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[[Image:1r5n.png|left|200px]]
 
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==Crystal Structure Analysis of sup35 complexed with GDP==
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The line below this paragraph, containing "STRUCTURE_1r5n", creates the "Structure Box" on the page.
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<StructureSection load='1r5n' size='340' side='right'caption='[[1r5n]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1r5n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R5N FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
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{{STRUCTURE_1r5n| PDB=1r5n | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r5n OCA], [https://pdbe.org/1r5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r5n RCSB], [https://www.ebi.ac.uk/pdbsum/1r5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r5n ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ERF3_SCHPO ERF3_SCHPO] Involved in translation termination. Stimulates the activity of ERF1. Binds guanine nucleotides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/1r5n_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r5n ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Translation termination in eukaryotes is governed by two interacting release factors, eRF1 and eRF3. The crystal structure of the eEF1alpha-like region of eRF3 from S. pombe determined in three states (free protein, GDP-, and GTP-bound forms) reveals an overall structure that is similar to EF-Tu, although with quite different domain arrangements. In contrast to EF-Tu, GDP/GTP binding to eRF3c does not induce dramatic conformational changes, and Mg(2+) is not required for GDP binding to eRF3c. Mg(2+) at higher concentration accelerates GDP release, suggesting a novel mechanism for nucleotide exchange on eRF3 from that of other GTPases. Mapping sequence conservation onto the molecular surface, combined with mutagenesis analysis, identified the eRF1 binding region, and revealed an essential function for the C terminus of eRF3. The N-terminal extension, rich in acidic amino acids, blocks the proposed eRF1 binding site, potentially regulating eRF1 binding to eRF3 in a competitive manner.
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===Crystal Structure Analysis of sup35 complexed with GDP===
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Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe.,Kong C, Ito K, Walsh MA, Wada M, Liu Y, Kumar S, Barford D, Nakamura Y, Song H Mol Cell. 2004 Apr 23;14(2):233-45. PMID:15099522<ref>PMID:15099522</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15099522}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1r5n" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15099522 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15099522}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1R5N is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R5N OCA].
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==Reference==
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Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 from S. pombe., Kong C, Ito K, Walsh MA, Wada M, Liu Y, Kumar S, Barford D, Nakamura Y, Song H, Mol Cell. 2004 Apr 23;14(2):233-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15099522 15099522]
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[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
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[[Category: Single protein]]
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[[Category: Kong C]]
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[[Category: Kong, C.]]
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[[Category: Song H]]
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[[Category: Song, H.]]
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[[Category: Gtpase]]
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[[Category: Peptide release]]
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[[Category: Translation termination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:06:49 2008''
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Current revision

Crystal Structure Analysis of sup35 complexed with GDP

PDB ID 1r5n

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