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1q4q

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(New page: 200px<br /><applet load="1q4q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q4q, resolution 2.1&Aring;" /> '''Crystal structure of ...)
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[[Image:1q4q.gif|left|200px]]<br /><applet load="1q4q" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q4q, resolution 2.1&Aring;" />
 
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'''Crystal structure of a DIAP1-Dronc complex'''<br />
 
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==Overview==
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==Crystal structure of a DIAP1-Dronc complex==
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The inhibitor of apoptosis protein DIAP1 inhibits Dronc-dependent cell, death by ubiquitinating Dronc. The pro-death proteins Reaper, Hid and Grim, (RHG) promote apoptosis by antagonizing DIAP1 function. Here we report the, structural basis of Dronc recognition by DIAP1 as well as a novel, mechanism by which the RHG proteins remove DIAP1-mediated downregulation, of Dronc. Biochemical and structural analyses revealed that the second BIR, (BIR2) domain of DIAP1 recognizes a 12-residue sequence in Dronc. This, recognition is essential for DIAP1 binding to Dronc, and for targeting, Dronc for ubiquitination. Notably, the Dronc-binding surface on BIR2, coincides with that required for binding to the N termini of the RHG, proteins, which competitively eliminate DIAP1-mediated ubiquitination of, Dronc. These observations reveal the molecular mechanisms of how DIAP1, recognizes Dronc, and more importantly, how the RHG proteins remove, DIAP1-mediated ubiquitination of Dronc.
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<StructureSection load='1q4q' size='340' side='right'caption='[[1q4q]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1q4q]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q4Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q4Q FirstGlance]. <br>
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1Q4Q is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q4Q OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q4q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q4q OCA], [https://pdbe.org/1q4q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q4q RCSB], [https://www.ebi.ac.uk/pdbsum/1q4q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q4q ProSAT]</span></td></tr>
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Molecular mechanism of Reaper-Grim-Hid-mediated suppression of DIAP1-dependent Dronc ubiquitination., Chai J, Yan N, Huh JR, Wu JW, Li W, Hay BA, Shi Y, Nat Struct Biol. 2003 Nov;10(11):892-8. Epub 2003 Sep 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14517550 14517550]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DIAP1_DROME DIAP1_DROME] Anti-apoptotic protein which functions as a caspase regulator, using its E3 ubiquitin-protein ligase activity to smother caspase activity. Binds, ubiquitinates and inactivates initiator caspase Dronc, and effector caspases Drice and Dcp-1. Acts as a Nedd8-E3 ubiquitin-protein ligase for Drice. Suppresses apoptosis by targeting the apoptosome for ubiquitination and inactivation. Plays an important role in cell motility. Overexpression suppresses rpr and hid-dependent cell death in the eye. Interaction of Diap1 with Dronc is required to suppress Dronc-mediated cell death through Diap1-mediated ubiquitination of Dronc. Acts as a positive regulator of Wnt signaling.<ref>PMID:14517550</ref> <ref>PMID:17397804</ref> <ref>PMID:18259196</ref> <ref>PMID:21145488</ref> <ref>PMID:22304967</ref> <ref>PMID:8548811</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q4/1q4q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q4q ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Chai, J.]]
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[[Category: Chai J]]
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[[Category: Shi, Y.]]
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[[Category: Shi Y]]
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[[Category: Yan, N.]]
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[[Category: Yan N]]
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[[Category: ZN]]
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[[Category: apoptosis]]
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[[Category: caspase]]
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[[Category: iap]]
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[[Category: mechanism]]
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[[Category: ubiquitination]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:23:03 2007''
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Current revision

Crystal structure of a DIAP1-Dronc complex

PDB ID 1q4q

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