1q5d

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(New page: 200px<br /><applet load="1q5d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q5d, resolution 1.93&Aring;" /> '''Epothilone B-bound C...)
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[[Image:1q5d.jpg|left|200px]]<br /><applet load="1q5d" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q5d, resolution 1.93&Aring;" />
 
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'''Epothilone B-bound Cytochrome P450epoK'''<br />
 
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==Overview==
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==Epothilone B-bound Cytochrome P450epoK==
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Epothilones are potential anticancer drugs that stabilize microtubules by, binding to tubulin in a manner similar to paclitaxel. Cytochrome P450epoK, (P450epoK), a heme containing monooxygenase involved in epothilone, biosynthesis in the myxobacterium Sorangium cellulosum, catalyzes the, epoxidation of epothilones C and D into epothilones A and B, respectively., The 2.10-, 1.93-, and 2.65-A crystal structures reported here for the, epothilone D-bound, epothilone B-bound, and substrate-free forms, respectively, are the first crystal structures of an epothilone-binding, protein. Although the substrate for P450epoK is the largest of a P450, whose x-ray structure is known, the structural changes along with, substrate binding or product release are very minor and the overall fold, is similar to other P450s. The epothilones are positioned with the, macrolide ring roughly perpendicular to the heme plane and I helix, and, the thiazole moiety provides key interactions that very likely are, critical in determining substrate specificity. Interestingly, there are, strong parallels between the epothilone/P450epoK and paclitaxel/tubulin, interactions. Based on structural similarities, a plausible epothilone, tubulin-binding mode is proposed.
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<StructureSection load='1q5d' size='340' side='right'caption='[[1q5d]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1q5d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorangium_cellulosum Sorangium cellulosum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q5D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPB:7,11-DIHYDROXY-8,8,10,12,16-PENTAMETHYL-3-[1-METHYL-2-(2-METHYL-THIAZOL-4-YL)VINYL]-4,17-DIOXABICYCLO[14.1.0]HEPTADECANE-5,9-DIONE'>EPB</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q5d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q5d OCA], [https://pdbe.org/1q5d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q5d RCSB], [https://www.ebi.ac.uk/pdbsum/1q5d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q5d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/C167_SORCE C167_SORCE] Involved in the biosynthesis of epothilones, macrolactones which have a narrow anti-fungal spectrum and microtubule-stabilizing activity. Responsible for the epooxidation of epothilones C and D to epothilones A and B, respectively.<ref>PMID:10831849</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q5/1q5d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q5d ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Q5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sorangium_cellulosum Sorangium cellulosum] with HEM and EPB as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q5D OCA].
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structures of epothilone D-bound, epothilone B-bound, and substrate-free forms of cytochrome P450epoK., Nagano S, Li H, Shimizu H, Nishida C, Ogura H, Ortiz de Montellano PR, Poulos TL, J Biol Chem. 2003 Nov 7;278(45):44886-93. Epub 2003 Aug 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12933799 12933799]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sorangium cellulosum]]
[[Category: Sorangium cellulosum]]
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[[Category: Li, H.]]
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[[Category: Li H]]
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[[Category: Montellano, P.R.Ortiz.de.]]
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[[Category: Nagano S]]
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[[Category: Nagano, S.]]
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[[Category: Nishida C]]
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[[Category: Nishida, C.]]
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[[Category: Ogura H]]
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[[Category: Ogura, H.]]
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[[Category: Ortiz de Montellano PR]]
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[[Category: Poulos, T.L.]]
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[[Category: Poulos TL]]
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[[Category: Shimizu, H.]]
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[[Category: Shimizu H]]
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[[Category: EPB]]
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[[Category: HEM]]
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[[Category: cytochrome p450]]
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[[Category: epothilone]]
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[[Category: heme-enzyme]]
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[[Category: oxydoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:23:43 2007''
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Current revision

Epothilone B-bound Cytochrome P450epoK

PDB ID 1q5d

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