1q7f

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(New page: 200px<br /><applet load="1q7f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q7f, resolution 1.95&Aring;" /> '''Brain Tumor NHL doma...)
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[[Image:1q7f.jpg|left|200px]]<br /><applet load="1q7f" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q7f, resolution 1.95&Aring;" />
 
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'''Brain Tumor NHL domain'''<br />
 
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==Overview==
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==Brain Tumor NHL domain==
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The Brain Tumor (Brat) protein is recruited to the 3' untranslated region, (UTR) of hunchback mRNA to regulate its translation. Recruitment is, mediated by interactions between the Pumilio RNA-binding Puf repeats and, the NHL domain of Brat, a conserved structural motif present in a large, family of growth regulators. In this report, we describe the crystal, structure of the Brat NHL domain and present a model of the Pumilio-Brat, complex derived from in silico docking experiments and supported by, mutational analysis of the protein-protein interface. A key feature of the, model is recognition of the outer, convex surface of the Pumilio Puf, domain by the top, electropositive face of the six-bladed Brat, beta-propeller. In particular, an extended loop in Puf repeat 8 fits in, the entrance to the central channel of the Brat beta-propeller. Together, these interactions are likely to be prototypic of the recruitment, strategies of other NHL-containing proteins in development.
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<StructureSection load='1q7f' size='340' side='right'caption='[[1q7f]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1q7f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q7F FirstGlance]. <br>
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1Q7F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q7F OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q7f OCA], [https://pdbe.org/1q7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q7f RCSB], [https://www.ebi.ac.uk/pdbsum/1q7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q7f ProSAT]</span></td></tr>
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==Reference==
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</table>
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Model of the brain tumor-Pumilio translation repressor complex., Edwards TA, Wilkinson BD, Wharton RP, Aggarwal AK, Genes Dev. 2003 Oct 15;17(20):2508-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14561773 14561773]
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== Function ==
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[https://www.uniprot.org/uniprot/BRAT_DROME BRAT_DROME] Plays a central role in translation repression of hb mRNA by being recruited by nos and pum to the Nanos Response Element (NRE), a 16 bp sequence in the hb mRNA 3'-UTR. Probably recruited by other proteins to repress translation of other mRNAs in other tissues. Involved in the regulation of ribosomal RNA synthesis and cell growth. Participates in abdominal segmentation and imaginal disk development. Involved in brain tumor suppression, by acting as a growth suppressor in the larval brain.<ref>PMID:11274060</ref> <ref>PMID:11807032</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q7/1q7f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q7f ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aggarwal, A.K.]]
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[[Category: Aggarwal AK]]
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[[Category: Edwards, T.A.]]
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[[Category: Edwards TA]]
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[[Category: Wharton, R.P.]]
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[[Category: Wharton RP]]
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[[Category: Wilkinson, B.D.]]
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[[Category: Wilkinson BD]]
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[[Category: beta-propeller]]
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[[Category: brat]]
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[[Category: nhl domain]]
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[[Category: nhl repeat]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:27:08 2007''
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Current revision

Brain Tumor NHL domain

PDB ID 1q7f

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