1q90

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(New page: 200px<br /><applet load="1q90" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q90, resolution 3.10&Aring;" /> '''Structure of the cyt...)
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[[Image:1q90.gif|left|200px]]<br /><applet load="1q90" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1q90, resolution 3.10&Aring;" />
 
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'''Structure of the cytochrome b6f (plastohydroquinone : plastocyanin oxidoreductase) from Chlamydomonas reinhardtii'''<br />
 
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==Overview==
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==Structure of the cytochrome b6f (plastohydroquinone : plastocyanin oxidoreductase) from Chlamydomonas reinhardtii==
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Photosystems I and II (PSI and II) are reaction centres that capture light, energy in order to drive oxygenic photosynthesis; however, they can only, do so by interacting with the multisubunit cytochrome b(6)f complex. This, complex receives electrons from PSII and passes them to PSI, pumping, protons across the membrane and powering the Q-cycle. Unlike the, mitochondrial and bacterial homologue cytochrome bc(1), cytochrome b(6)f, can switch to a cyclic mode of electron transfer around PSI using an, unknown pathway. Here we present the X-ray structure at 3.1 A of, cytochrome b(6)f from the alga Chlamydomonas reinhardtii. The structure, bears similarities to cytochrome bc(1) but also exhibits some unique, features, such as binding chlorophyll, beta-carotene and an unexpected, haem sharing a quinone site. This haem is atypical as it is covalently, bound by one thioether linkage and has no axial amino acid ligand. This, haem may be the missing link in oxygenic photosynthesis.
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<StructureSection load='1q90' size='340' side='right'caption='[[1q90]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1q90]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q90 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCR:BETA-CAROTENE'>BCR</scene>, <scene name='pdbligand=CLA:CHLOROPHYLL+A'>CLA</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=LFA:EICOSANE'>LFA</scene>, <scene name='pdbligand=LMG:1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE'>LMG</scene>, <scene name='pdbligand=SQD:1,2-DI-O-ACYL-3-O-[6-DEOXY-6-SULFO-ALPHA-D-GLUCOPYRANOSYL]-SN-GLYCEROL'>SQD</scene>, <scene name='pdbligand=TDS:8-HYDROXY-5,7-DIMETHOXY-3-METHYL-2-TRIDECYL-4H-CHROMEN-4-ONE'>TDS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q90 OCA], [https://pdbe.org/1q90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q90 RCSB], [https://www.ebi.ac.uk/pdbsum/1q90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q90 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYF_CHLRE CYF_CHLRE] Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.[HAMAP-Rule:MF_00610]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q9/1q90_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1q90 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Photosystems I and II (PSI and II) are reaction centres that capture light energy in order to drive oxygenic photosynthesis; however, they can only do so by interacting with the multisubunit cytochrome b(6)f complex. This complex receives electrons from PSII and passes them to PSI, pumping protons across the membrane and powering the Q-cycle. Unlike the mitochondrial and bacterial homologue cytochrome bc(1), cytochrome b(6)f can switch to a cyclic mode of electron transfer around PSI using an unknown pathway. Here we present the X-ray structure at 3.1 A of cytochrome b(6)f from the alga Chlamydomonas reinhardtii. The structure bears similarities to cytochrome bc(1) but also exhibits some unique features, such as binding chlorophyll, beta-carotene and an unexpected haem sharing a quinone site. This haem is atypical as it is covalently bound by one thioether linkage and has no axial amino acid ligand. This haem may be the missing link in oxygenic photosynthesis.
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==About this Structure==
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An atypical haem in the cytochrome b(6)f complex.,Stroebel D, Choquet Y, Popot JL, Picot D Nature. 2003 Nov 27;426(6965):413-8. PMID:14647374<ref>PMID:14647374</ref>
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1Q90 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] with HEM, FES, CL1, BCR, TDS, SQD, LFA and LMG as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Plastoquinol--plastocyanin_reductase Plastoquinol--plastocyanin reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.99.1 1.10.99.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Q90 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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An atypical haem in the cytochrome b(6)f complex., Stroebel D, Choquet Y, Popot JL, Picot D, Nature. 2003 Nov 27;426(6965):413-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14647374 14647374]
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</div>
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[[Category: Chlamydomonas reinhardtii]]
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<div class="pdbe-citations 1q90" style="background-color:#fffaf0;"></div>
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[[Category: Plastoquinol--plastocyanin reductase]]
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[[Category: Protein complex]]
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[[Category: Choquet, Y.]]
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[[Category: Picot, D.]]
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[[Category: Popot, J.L.]]
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[[Category: Stroebel, D.]]
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[[Category: BCR]]
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[[Category: CL1]]
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[[Category: FES]]
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[[Category: HEM]]
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[[Category: LFA]]
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[[Category: LMG]]
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[[Category: SQD]]
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[[Category: TDS]]
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[[Category: beta-carotene]]
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[[Category: chlorophyll]]
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[[Category: electron transfer]]
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[[Category: membrane protein complex]]
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[[Category: monogalactosyldiacylglycerol]]
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[[Category: oxydoreductase]]
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[[Category: photosynthesis]]
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[[Category: stigmatellin]]
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[[Category: sulfoquinovosyldiacylglycerol]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:29:52 2007''
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==See Also==
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*[[Cytochrome b6|Cytochrome b6]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Chlamydomonas reinhardtii]]
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[[Category: Large Structures]]
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[[Category: Choquet Y]]
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[[Category: Picot D]]
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[[Category: Popot J-L]]
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[[Category: Stroebel D]]

Current revision

Structure of the cytochrome b6f (plastohydroquinone : plastocyanin oxidoreductase) from Chlamydomonas reinhardtii

PDB ID 1q90

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