1uij

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{{Seed}}
 
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[[Image:1uij.png|left|200px]]
 
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==Crystal Structure Of Soybean beta-Conglycinin Beta Homotrimer (I122M/K124W)==
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The line below this paragraph, containing "STRUCTURE_1uij", creates the "Structure Box" on the page.
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<StructureSection load='1uij' size='340' side='right'caption='[[1uij]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1uij]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UIJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uij OCA], [https://pdbe.org/1uij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uij RCSB], [https://www.ebi.ac.uk/pdbsum/1uij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uij ProSAT]</span></td></tr>
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{{STRUCTURE_1uij| PDB=1uij | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLCB1_SOYBN GLCB1_SOYBN] Seed storage protein. Accumulates during seed development and is hydrolyzed after germination to provide a carbon and nitrogen source for the developing seedling.<ref>PMID:2562562</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ui/1uij_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uij ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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beta-Conglycinin is composed of three kinds of subunit: alpha, alpha' and beta. A phagocytosis-stimulating peptide sequence (MITLAIPVNKPGR), soymetide, exists in the alpha' subunit of beta-conglycinin. Met at N terminus of the soymetide is essential for the activity. When Thr at the third residue from N terminus of the soymetide is replaced by Phe or Trp, the phagocytosis-stimulating activity greatly increases (Thr&lt;Phe&lt;Trp). The beta subunit does not exhibit the phagocytosis-stimulating activity because the residues corresponding to the first and third residues in the soymetide are Ile and Lys, respectively. In this study, we introduced the phagocytosis-stimulating peptide sequence (Ile--&gt;Met, Lys--&gt;Thr, Phe, or Trp) into the beta subunit after confirmation of the effects of residue replacements by molecular modeling, suggesting that the introduced mutations might not prevent the correct folding. The studies of circular dichroism (CD), gel filtration and differential scanning calorimetry (DSC) of the mutants (I122M/K124T, I122M/K124F, I122M/K124W) expressed in E. coli demonstrated that they folded and self-assembled similarly to the wild type. This was confirmed by X-ray analysis of I122M/K124W crystal where the biggest residue tryptophane was introduced. The three mutants exhibited phagocytosis activities after digestion by trypsin, and the order was the wild type&lt;I122M/K124T&lt;I122M/K124F&lt;I122M/K124W as expected.
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===Crystal Structure Of Soybean beta-Conglycinin Beta Homotrimer (I122M/K124W)===
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Creation of soybean beta-conglycinin beta with strong phagocytosis-stimulating activity.,Maruyama N, Maruyama Y, Tsuruki T, Okuda E, Yoshikawa M, Utsumi S Biochim Biophys Acta. 2003 May 30;1648(1-2):99-104. PMID:12758152<ref>PMID:12758152</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12758152}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1uij" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12758152 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12758152}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1UIJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UIJ OCA].
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==Reference==
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Creation of soybean beta-conglycinin beta with strong phagocytosis-stimulating activity., Maruyama N, Maruyama Y, Tsuruki T, Okuda E, Yoshikawa M, Utsumi S, Biochim Biophys Acta. 2003 May 30;1648(1-2):99-104. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12758152 12758152]
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[[Category: Glycine max]]
[[Category: Glycine max]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Maruyama, N.]]
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[[Category: Maruyama N]]
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[[Category: Maruyama, Y.]]
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[[Category: Maruyama Y]]
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[[Category: Okuda, E.]]
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[[Category: Okuda E]]
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[[Category: Tsuruki, T.]]
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[[Category: Tsuruki T]]
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[[Category: Utsumi, S.]]
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[[Category: Utsumi S]]
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[[Category: Yoshikawa, M.]]
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[[Category: Yoshikawa M]]
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[[Category: Double-stranded beta helix]]
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[[Category: Seed storage protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:29:25 2008''
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Current revision

Crystal Structure Of Soybean beta-Conglycinin Beta Homotrimer (I122M/K124W)

PDB ID 1uij

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