1qcq

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(New page: 200px<br /><applet load="1qcq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qcq, resolution 2.7&Aring;" /> '''UBIQUITIN CONJUGATING...)
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[[Image:1qcq.gif|left|200px]]<br /><applet load="1qcq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qcq, resolution 2.7&Aring;" />
 
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'''UBIQUITIN CONJUGATING ENZYME'''<br />
 
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==Overview==
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==UBIQUITIN CONJUGATING ENZYME==
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The three-dimensional structure of a yeast ubiquitin-conjugating enzyme, encoded by the Saccharomyces cerevisiae UBC4 gene, has been determined at, 2.7 A. The structure was solved using molecular replacement techniques and, refined by simulated annealing to an R-factor of 0.198. Bond lengths and, angles in the molecule have root mean square deviations from ideal values, of 0.018 A and 4.0 degrees, respectively. Ubc4 is an alpha/beta protein, with four alpha-helices and a four-stranded antiparallel beta-sheet. The, ubiquitin-accepting cysteine is located in a cleft between two loops., Comparison with the recently determined structure of a different plant, enzyme suggests that class I ubiquitin-conjugating enzymes are highly, conserved in their three-dimensional folding. Except for two extra, residues at the N- and the C-terminus of the plant enzyme, the C alpha, atoms of the two enzymes can be superimposed with a root mean square, deviation of only 1.52 A. Greater variations are found between the, surfaces of the two molecules, as most of the identical residues between, the two enzymes are either buried or clustered on the surface that lies, adjacent to the ubiquitin-accepting cysteine. We suggest that this, conserved surface functions in protein-protein binding during ubiquitin, thiol ester formation.
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<StructureSection load='1qcq' size='340' side='right'caption='[[1qcq]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qcq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2uce 2uce]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QCQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qcq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qcq OCA], [https://pdbe.org/1qcq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qcq RCSB], [https://www.ebi.ac.uk/pdbsum/1qcq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qcq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UBC4_YEAST UBC4_YEAST] Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Mediates ubiquitination of PEX5.<ref>PMID:17550898</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qc/1qcq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qcq ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1QCQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. This structure superseeds the now removed PDB entry 2UCE. Active as [http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QCQ OCA].
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*[[3D structures of ubiquitin conjugating enzyme|3D structures of ubiquitin conjugating enzyme]]
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== References ==
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==Reference==
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<references/>
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Tertiary structures of class I ubiquitin-conjugating enzymes are highly conserved: crystal structure of yeast Ubc4., Cook WJ, Jeffrey LC, Xu Y, Chau V, Biochemistry. 1993 Dec 21;32(50):13809-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8268156 8268156]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Chau V]]
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[[Category: Ubiquitin--protein ligase]]
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[[Category: Cook WJ]]
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[[Category: Chau, V.]]
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[[Category: Jeffrey LC]]
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[[Category: Cook, W.J.]]
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[[Category: Xu Y]]
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[[Category: Jeffrey, L.C.]]
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[[Category: Xu, Y.]]
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[[Category: ubiquitin]]
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[[Category: ubiquitin-conjugating enzyme]]
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[[Category: yeast]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:34:39 2007''
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Current revision

UBIQUITIN CONJUGATING ENZYME

PDB ID 1qcq

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