1qcs

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(New page: 200px<br /><applet load="1qcs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qcs, resolution 1.90&Aring;" /> '''N-TERMINAL DOMAIN OF...)
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[[Image:1qcs.jpg|left|200px]]<br /><applet load="1qcs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qcs, resolution 1.90&Aring;" />
 
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'''N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)'''<br />
 
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==Overview==
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==N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)==
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N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential, for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles, cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans, complex formation. We have determined the crystal structure of the, N-terminal domain of NSF (N) to 1.9 A resolution. N contains two, subdomains which form a groove that is a likely SNAP interaction site., Unexpectedly, both N subdomains are structurally similar to domains in, EF-Tu. Based on this similarity, we propose a model for a large, conformational change in NSF that drives SNARE complex disassembly.
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<StructureSection load='1qcs' size='340' side='right'caption='[[1qcs]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qcs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QCS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qcs OCA], [https://pdbe.org/1qcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qcs RCSB], [https://www.ebi.ac.uk/pdbsum/1qcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qcs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NSF_CRIGR NSF_CRIGR] Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qc/1qcs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qcs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans complex formation. We have determined the crystal structure of the N-terminal domain of NSF (N) to 1.9 A resolution. N contains two subdomains which form a groove that is a likely SNAP interaction site. Unexpectedly, both N subdomains are structurally similar to domains in EF-Tu. Based on this similarity, we propose a model for a large conformational change in NSF that drives SNARE complex disassembly.
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==About this Structure==
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NSF N-terminal domain crystal structure: models of NSF function.,Yu RC, Jahn R, Brunger AT Mol Cell. 1999 Jul;4(1):97-107. PMID:10445031<ref>PMID:10445031</ref>
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1QCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QCS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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NSF N-terminal domain crystal structure: models of NSF function., Yu RC, Jahn R, Brunger AT, Mol Cell. 1999 Jul;4(1):97-107. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10445031 10445031]
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</div>
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<div class="pdbe-citations 1qcs" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Cricetulus griseus]]
[[Category: Cricetulus griseus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Brunger, A.T.]]
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[[Category: Brunger AT]]
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[[Category: Jahn, R.]]
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[[Category: Jahn R]]
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[[Category: Yu, R.C.]]
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[[Category: Yu RC]]
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[[Category: SO4]]
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[[Category: double-psi beta barrel alpha beta roll]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:34:52 2007''
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N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)

PDB ID 1qcs

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