1xrk

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{{Seed}}
 
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[[Image:1xrk.png|left|200px]]
 
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==Crystal structure of a mutant bleomycin binding protein from Streptoalloteichus hindustanus displaying increased thermostability==
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The line below this paragraph, containing "STRUCTURE_1xrk", creates the "Structure Box" on the page.
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<StructureSection load='1xrk' size='340' side='right'caption='[[1xrk]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xrk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptoalloteichus_hindustanus Streptoalloteichus hindustanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XRK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XRK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLM:BLEOMYCIN+A2'>BLM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1xrk| PDB=1xrk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xrk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xrk OCA], [https://pdbe.org/1xrk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xrk RCSB], [https://www.ebi.ac.uk/pdbsum/1xrk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xrk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BLE_STRHI BLE_STRHI] Binding protein with a strong affinity to the bleomycin family of antibiotics.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xr/1xrk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xrk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Limited thermostability of antibiotic resistance markers has restricted genetic research in the field of extremely thermophilic Archaea and bacteria. In this study, we used directed evolution and selection in the thermophilic bacterium Thermus thermophilus HB27 to find thermostable variants of a bleomycin-binding protein from the mesophilic bacterium Streptoalloteichus hindustanus. In a single selection round, we identified eight clones bearing five types of double mutated genes that provided T. thermophilus transformants with bleomycin resistance at 77 degrees C, while the wild-type gene could only do so up to 65 degrees C. Only six different amino acid positions were altered, three of which were glycine residues. All variant proteins were produced in Escherichia coli and analyzed biochemically for thermal stability and functionality at high temperature. A synthetic mutant resistance gene with low GC content was designed that combined four substitutions. The encoded protein showed up to 17 degrees C increased thermostability and unfolded at 85 degrees C in the absence of bleomycin, whereas in its presence the protein unfolded at 100 degrees C. Despite these highly thermophilic properties, this mutant was still able to function normally at mesophilic temperatures in vivo. The mutant protein was co-crystallized with bleomycin, and the structure of the binary complex was determined to a resolution of 1.5 A. Detailed structural analysis revealed possible molecular mechanisms of thermostabilization and enhanced antibiotic binding, which included the introduction of an intersubunit hydrogen bond network, improved hydrophobic packing of surface indentations, reduction of loop flexibility, and alpha-helix stabilization. The potential applicability of the thermostable selection marker is discussed.
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===Crystal structure of a mutant bleomycin binding protein from Streptoalloteichus hindustanus displaying increased thermostability===
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Engineering a selectable marker for hyperthermophiles.,Brouns SJ, Wu H, Akerboom J, Turnbull AP, de Vos WM, van der Oost J J Biol Chem. 2005 Mar 25;280(12):11422-31. Epub 2005 Jan 7. PMID:15640151<ref>PMID:15640151</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15640151}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1xrk" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15640151 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15640151}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1XRK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptoalloteichus_hindustanus Streptoalloteichus hindustanus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XRK OCA].
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==Reference==
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Engineering a selectable marker for hyperthermophiles., Brouns SJ, Wu H, Akerboom J, Turnbull AP, de Vos WM, van der Oost J, J Biol Chem. 2005 Mar 25;280(12):11422-31. Epub 2005 Jan 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15640151 15640151]
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[[Category: Single protein]]
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[[Category: Streptoalloteichus hindustanus]]
[[Category: Streptoalloteichus hindustanus]]
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[[Category: Akerboom, J.]]
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[[Category: Akerboom J]]
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[[Category: Brouns, S J.J.]]
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[[Category: Brouns SJJ]]
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[[Category: Oost, J Van der.]]
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[[Category: Turnbull AP]]
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[[Category: Turnbull, A P.]]
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[[Category: Van der Oost J]]
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[[Category: Vos, W M.de.]]
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[[Category: Wu H]]
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[[Category: Wu, H.]]
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[[Category: De Vos WM]]
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[[Category: Arm exchange]]
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[[Category: Ligand binding protein]]
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[[Category: Thermostable mutant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:39:45 2008''
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Current revision

Crystal structure of a mutant bleomycin binding protein from Streptoalloteichus hindustanus displaying increased thermostability

PDB ID 1xrk

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