1wky

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{{Seed}}
 
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[[Image:1wky.png|left|200px]]
 
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==Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module==
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The line below this paragraph, containing "STRUCTURE_1wky", creates the "Structure Box" on the page.
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<StructureSection load='1wky' size='340' side='right'caption='[[1wky]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1wky]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._JAMB-602 Bacillus sp. JAMB-602]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WKY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_1wky| PDB=1wky | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wky OCA], [https://pdbe.org/1wky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wky RCSB], [https://www.ebi.ac.uk/pdbsum/1wky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wky ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q4W8M3_9BACI Q4W8M3_9BACI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wk/1wky_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wky ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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An alkaline mannanase (EC 3.2.1.78) from the alkaliphilic Bacillus sp. strain JAMB-602 was cloned and sequenced. The deduced amino-acid sequence of the enzyme suggested that the enzyme consists of a catalytic and unknown additional domains. The recombinant enzyme expressed by B. subtilis was crystallized using the hanging-drop vapour-diffusion method at 277 K. X-ray diffraction data were collected to 1.65 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.7, b = 79.5, c = 80.4 A. The asymmetric unit contains one protein molecule, with a corresponding VM of 2.26 A3 Da(-1) and a solvent content of 45.6%. Molecular replacement for initial phasing was carried out using the three-dimensional structure of a mannanase from Thermomonospora fusca as a search model, which corresponds to the catalytic domain of the alkaline mannanase. It gave sufficient phases to build the unknown domain.
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===Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module===
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Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate.,Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:15272186<ref>PMID:15272186</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15272186}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1wky" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15272186 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15272186}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Bacillus sp. JAMB-602]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WKY OCA].
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[[Category: Large Structures]]
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[[Category: Akita M]]
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==Reference==
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[[Category: Grant WD]]
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Crystallization and preliminary X-ray study of alkaline mannanase from an alkaliphilic Bacillus isolate., Akita M, Takeda N, Hirasawa K, Sakai H, Kawamoto M, Yamamoto M, Grant WD, Hatada Y, Ito S, Horikoshi K, Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1490-2. Epub 2004, Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15272186 15272186]
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[[Category: Hatada Y]]
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[[Category: Mannan endo-1,4-beta-mannosidase]]
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[[Category: Hirasawa K]]
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[[Category: Akita, M.]]
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[[Category: Horikoshi K]]
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[[Category: Grant, W D.]]
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[[Category: Ito S]]
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[[Category: Hatada, Y.]]
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[[Category: Kawamoto M]]
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[[Category: Hirasawa, K.]]
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[[Category: Sakai H]]
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[[Category: Horikoshi, K.]]
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[[Category: Takeda N]]
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[[Category: Ito, S.]]
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[[Category: Yamamoto M]]
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[[Category: Kawamoto, M.]]
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[[Category: Sakai, H.]]
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[[Category: Takeda, N.]]
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[[Category: Yamamoto, M.]]
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[[Category: Catalytic domain]]
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[[Category: Cbm]]
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[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:49:50 2008''
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Current revision

Crystal structure of alkaline mannanase from Bacillus sp. strain JAMB-602: catalytic domain and its Carbohydrate Binding Module

PDB ID 1wky

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