1qg5

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(New page: 200px<br /><applet load="1qg5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qg5, resolution 2.0&Aring;" /> '''HIGH RESOLUTION CRYST...)
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[[Image:1qg5.gif|left|200px]]<br /><applet load="1qg5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qg5, resolution 2.0&Aring;" />
 
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'''HIGH RESOLUTION CRYSTAL STRUCTURE OF THE BOVINE BETA-LACTOGLOBULIN (ISOFORM A)'''<br />
 
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==Overview==
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==HIGH RESOLUTION CRYSTAL STRUCTURE OF THE BOVINE BETA-LACTOGLOBULIN (ISOFORM A)==
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The crystal structures of beta-lactoglobulin genetic variants A and B have, been determined in the orthorhombic space group C222(1) (lattice Y) by, X-ray diffraction at 2.0 A and 1.95 A resolution, respectively. The, structural comparison shows that both variants exhibit the open, conformation of the EF loop at the pH of crystallization (pH 7.9), in, contrast to what has been reported for the same genetic variants at pH 7.1, in the trigonal space group P3221 (lattice Z) [Qin, B.Y., Bewley, M.C., Creamer, L.K., Baker, E.N. &amp; Jameson, G.B. (1999) Protein Sci. 8, 75-83]., Furthermore, it was found that the stereochemical environment of Tyr42, changes significantly with pH variation between pH 7 and pH 8. This may, provide a structural explanation for an as yet unexplained feature of the, Tanford transition, namely the increase in exposure of a tyrosine residue.
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<StructureSection load='1qg5' size='340' side='right'caption='[[1qg5]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qg5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QG5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QG5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qg5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qg5 OCA], [https://pdbe.org/1qg5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qg5 RCSB], [https://www.ebi.ac.uk/pdbsum/1qg5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qg5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qg/1qg5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qg5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structures of beta-lactoglobulin genetic variants A and B have been determined in the orthorhombic space group C222(1) (lattice Y) by X-ray diffraction at 2.0 A and 1.95 A resolution, respectively. The structural comparison shows that both variants exhibit the open conformation of the EF loop at the pH of crystallization (pH 7.9), in contrast to what has been reported for the same genetic variants at pH 7.1 in the trigonal space group P3221 (lattice Z) [Qin, B.Y., Bewley, M.C., Creamer, L.K., Baker, E.N. &amp; Jameson, G.B. (1999) Protein Sci. 8, 75-83]. Furthermore, it was found that the stereochemical environment of Tyr42 changes significantly with pH variation between pH 7 and pH 8. This may provide a structural explanation for an as yet unexplained feature of the Tanford transition, namely the increase in exposure of a tyrosine residue.
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==About this Structure==
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Crystal structures of bovine beta-lactoglobulin in the orthorhombic space group C222(1). Structural differences between genetic variants A and B and features of the Tanford transition.,Oliveira KM, Valente-Mesquita VL, Botelho MM, Sawyer L, Ferreira ST, Polikarpov I Eur J Biochem. 2001 Jan;268(2):477-83. PMID:11168385<ref>PMID:11168385</ref>
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1QG5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QG5 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of bovine beta-lactoglobulin in the orthorhombic space group C222(1). Structural differences between genetic variants A and B and features of the Tanford transition., Oliveira KM, Valente-Mesquita VL, Botelho MM, Sawyer L, Ferreira ST, Polikarpov I, Eur J Biochem. 2001 Jan;268(2):477-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11168385 11168385]
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</div>
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[[Category: Bos taurus]]
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<div class="pdbe-citations 1qg5" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Oliveira, K.M.G.]]
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[[Category: Polikarpov, I.]]
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[[Category: Sawyer, L.]]
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[[Category: isoform a]]
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[[Category: lipocalin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:39:39 2007''
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==See Also==
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*[[Beta-lactoglobulin 3D structures|Beta-lactoglobulin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Large Structures]]
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[[Category: Oliveira KMG]]
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[[Category: Polikarpov I]]
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[[Category: Sawyer L]]

Current revision

HIGH RESOLUTION CRYSTAL STRUCTURE OF THE BOVINE BETA-LACTOGLOBULIN (ISOFORM A)

PDB ID 1qg5

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