1qil

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(New page: 200px<br /><applet load="1qil" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qil, resolution 2.5&Aring;" /> '''INACTIVE MUTANT TOXIC...)
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[[Image:1qil.gif|left|200px]]<br /><applet load="1qil" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qil, resolution 2.5&Aring;" />
 
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'''INACTIVE MUTANT TOXIC SHOCK SYNDROME TOXIN-1 AT 2.5 A'''<br />
 
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==Overview==
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==INACTIVE MUTANT TOXIC SHOCK SYNDROME TOXIN-1 AT 2.5 A==
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Toxic shock syndrome toxin-1 (TSST-1) is one of a family of staphylococcal, exotoxins recognized as microbial superantigens. The toxin plays a, dominant role in the genesis of toxic shock in humans through a massive, activation of the immune system. This potentially lethal illness occurs as, a result of the interaction of TSST-1 with a significant proportion of the, T-cell repertoire. TSST-1, like other superantigens, can bind directly to, class II major histocompatibility (MHC class II) molecules prior to its, interaction with entire families of V beta chains of the T-cell receptor, (TCR). The three-dimensional structure of a mutant (His-135-Ala) TSST-1, was compared with the structure of the native (wild-type) TSST-1 at 2.5 A, resolution. The replacement of His 135 of TSST-1 with an Ala residue, results in the loss of T-cell mitogenicity and toxicity in experimental, animals. This residue, postulated to be directly involved in the toxin-TCR, interactions, is located on the major helix alpha 2, which forms the, backbone of the molecule and is exposed to the solvent. In the molecular, structure of the mutant toxin, the helix alpha 2 remains unaltered, but, the His to Ala modification causes perturbations on the neighboring helix, alpha 1 by disrupting helix-helix interactions. Thus, the effects on TCR, binding of the His 135 residue could actually be mediated, wholly or in, part, by the alpha 1 helix.
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<StructureSection load='1qil' size='340' side='right'caption='[[1qil]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qil]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QIL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QIL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qil FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qil OCA], [https://pdbe.org/1qil PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qil RCSB], [https://www.ebi.ac.uk/pdbsum/1qil PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qil ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TSST_STAAU TSST_STAAU] Responsible for the symptoms of toxic shock syndrome.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qi/1qil_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qil ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1QIL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QIL OCA].
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*[[Acetylcholinesterase 3D structures|Acetylcholinesterase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of a biologically inactive mutant of toxic shock syndrome toxin-1 at 2.5 A resolution., Papageorgiou AC, Quinn CP, Beer D, Brehm RD, Tranter HS, Bonventre PF, Acharya KR, Protein Sci. 1996 Aug;5(8):1737-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8844860 8844860]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
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[[Category: Acharya, K.R.]]
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[[Category: Acharya KR]]
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[[Category: Papageorgiou, A.C.]]
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[[Category: Papageorgiou AC]]
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[[Category: staphylococcal enterotoxin]]
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[[Category: superantigen]]
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[[Category: toxin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:44:20 2007''
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Current revision

INACTIVE MUTANT TOXIC SHOCK SYNDROME TOXIN-1 AT 2.5 A

PDB ID 1qil

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