1qiu

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(New page: 200px<br /><applet load="1qiu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qiu, resolution 2.40&Aring;" /> '''A TRIPLE BETA-SPIRAL...)
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[[Image:1qiu.jpg|left|200px]]<br /><applet load="1qiu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qiu, resolution 2.40&Aring;" />
 
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'''A TRIPLE BETA-SPIRAL IN THE ADENOVIRUS FIBRE SHAFT REVEALS A NEW STRUCTURAL MOTIF FOR BIOLOGICAL FIBRES'''<br />
 
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==Overview==
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==A triple beta-spiral in the adenovirus fibre shaft reveals a new structural motif for biological fibres==
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Human adenoviruses are responsible for respiratory, gastroenteric and, ocular infections and can serve as gene therapy vectors. They form, icosahedral particles with 240 copies of the trimeric hexon protein, arranged on the planes and a penton complex at each of the twelve, vertices. The penton consists of a pentameric base, implicated in virus, internalization, and a protruding trimeric fibre, responsible for receptor, attachment. The fibres are homo-trimeric proteins containing an, amino-terminal penton base attachment domain, a long, thin central shaft, and a carboxy-terminal cell attachment or head domain. The shaft domain, contains a repeating sequence motif with an invariant glycine or proline, and a conserved pattern of hydrophobic residues. Here we describe the, crystal structure at 2.4 A resolution of a recombinant protein containing, the four distal repeats of the adenovirus type 2 fibre shaft plus the, receptor-binding head domain. The structure reveals a novel triple, beta-spiral fibrous fold for the shaft. Implications for folding of, fibrous proteins (misfolding of shaft peptides leads to amyloid-like, fibrils) and for the design of a new class of artificial, silk-like, fibrous materials are discussed.
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<StructureSection load='1qiu' size='340' side='right'caption='[[1qiu]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1qiu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_2 Human adenovirus 2]. The December 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Adenovirus'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_12 10.2210/rcsb_pdb/mom_2010_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QIU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QIU FirstGlance]. <br>
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1QIU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_2 Human adenovirus 2]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QIU OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qiu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qiu OCA], [https://pdbe.org/1qiu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qiu RCSB], [https://www.ebi.ac.uk/pdbsum/1qiu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qiu ProSAT]</span></td></tr>
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==Reference==
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</table>
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A triple beta-spiral in the adenovirus fibre shaft reveals a new structural motif for a fibrous protein., van Raaij MJ, Mitraki A, Lavigne G, Cusack S, Nature. 1999 Oct 28;401(6756):935-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10553913 10553913]
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== Function ==
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[https://www.uniprot.org/uniprot/SPIKE_ADE02 SPIKE_ADE02] Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host coxsackievirus and adenovirus receptor CXADR located at the cell tight junctions to provide virion initial attachment to target cell. The fiber protein binds to CXADR with a higher affinity than CXADR binds to itself, thereby blocking the cell-cell adhesion function of CXADR dimers and leading to local disruption of the tight junction. Fiber protein present on neo-synthesized particles may thus disrupt the junctional integrity in order to facilitate further neighboring cells infection. Fiber proteins are shed during virus entry, when virus is still at the cell surface. Fiber shedding is dependent on viral CXADR drifting motion and subsequent binding to immobile integrins. Heparan sulfate might also play a role in virus binding.<ref>PMID:10704346</ref> <ref>PMID:12297051</ref> <ref>PMID:21843868</ref> <ref>PMID:9525681</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qi/1qiu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qiu ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Adenovirus]]
[[Category: Human adenovirus 2]]
[[Category: Human adenovirus 2]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cusack, S.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Lavigne, G.]]
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[[Category: Cusack S]]
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[[Category: Mitraki, A.]]
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[[Category: Lavigne G]]
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[[Category: Raaij, M.J.Van.]]
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[[Category: Mitraki A]]
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[[Category: adenovirus]]
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[[Category: Van Raaij MJ]]
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[[Category: fibre protein]]
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[[Category: triple beta-spiral]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:44:42 2007''
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Current revision

A triple beta-spiral in the adenovirus fibre shaft reveals a new structural motif for biological fibres

PDB ID 1qiu

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