2gmr

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{{Seed}}
 
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[[Image:2gmr.png|left|200px]]
 
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==Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn==
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The line below this paragraph, containing "STRUCTURE_2gmr", creates the "Structure Box" on the page.
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<StructureSection load='2gmr' size='340' side='right'caption='[[2gmr]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2gmr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GMR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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{{STRUCTURE_2gmr| PDB=2gmr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmr OCA], [https://pdbe.org/2gmr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gmr RCSB], [https://www.ebi.ac.uk/pdbsum/2gmr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gmr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RCEL_CERSP RCEL_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gm/2gmr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gmr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The reaction center (RC) of Rhodobacter sphaeroides uses light energy to reduce and protonate a quinone molecule, QB (the secondary quinone electron acceptor), to form quinol, QBH2. Asp210 in the L-subunit has been shown to be a catalytic residue in this process. Mutation of Asp210 to Asn leads to a deceleration of reoxidation of QA- in the QA-QB --&gt; QAQB- transition. Here we determined the structure of the Asp210 to Asn mutant to 2.5 A and show that there are no major structural differences as compared to the wild-type protein. We found QB in the distal position and a chain of water molecules between Asn210 and QB. Using time-resolved Fourier transform infrared (trFTIR) spectroscopy, we characterized the molecular reaction mechanism of this mutant. We found that QB- formation precedes QA- oxidation even more pronounced than in the wild-type reaction center. Continuum absorbance changes indicate deprotonation of a protonated water cluster, most likely of the water chain between Asn210 and QB. A detailed analysis of wild-type structures revealed a highly conserved water chain between Asp210 or Glu210 and QB in Rb. sphaeroides and Rhodopseudomonas viridis, respectively.
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===Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn===
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Proton uptake in the reaction center mutant L210DN from Rhodobacter sphaeroides via protonated water molecules.,Hermes S, Stachnik JM, Onidas D, Remy A, Hofmann E, Gerwert K Biochemistry. 2006 Nov 21;45(46):13741-9. PMID:17105193<ref>PMID:17105193</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_17105193}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2gmr" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17105193 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17105193}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Cereibacter sphaeroides]]
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2GMR is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GMR OCA].
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[[Category: Large Structures]]
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[[Category: Gerwert K]]
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==Reference==
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[[Category: Hermes S]]
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Proton uptake in the reaction center mutant L210DN from Rhodobacter sphaeroides via protonated water molecules., Hermes S, Stachnik JM, Onidas D, Remy A, Hofmann E, Gerwert K, Biochemistry. 2006 Nov 21;45(46):13741-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17105193 17105193]
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[[Category: Hofmann E]]
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[[Category: Protein complex]]
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[[Category: Stachnik JM]]
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[[Category: Rhodobacter sphaeroides]]
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[[Category: Gerwert, K]]
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[[Category: Hermes, S.]]
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[[Category: Hofmann, E.]]
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[[Category: Stachnik, J M.]]
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[[Category: Electron transport]]
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[[Category: Integral membrane protein]]
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[[Category: L210dn]]
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[[Category: Mutant]]
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[[Category: Photosynthetic reaction center]]
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[[Category: Proton transfer]]
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[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 15:59:20 2008''
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Current revision

Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn

PDB ID 2gmr

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