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1qk7

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(New page: 200px<br /><applet load="1qk7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qk7" /> '''SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LE...)
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[[Image:1qk7.jpg|left|200px]]<br /><applet load="1qk7" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qk7" />
 
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'''SOLUTION STRUCTURE OF SELENOCOSMIA HUWENA LECTIN-I(SHL-I) BY 2D-NMR'''<br />
 
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==Overview==
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==Solution structure of Selenocosmia huwena lectin-I(SHL-I) by 2D-NMR==
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The three-dimensional structure of native SHL-I, a lectin from the venom, of the Chinese bird spider Selenocosmia huwena, has been determined from, two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best, 10 structures have NOE violation &lt;0.3 A, dihedral violation &lt;2 deg, and, average root-mean-square differences of 0.85 + 0.06 A over backbone atoms., The structure consists of a three-stranded antiparallel beta-sheet and, three turns. The three disulfide bridges and three-stranded antiparallel, beta-sheet form a inhibitor cystine knot motif which is adopted by several, other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin., The C-terminal fragment from Leu28 to Trp32 adopts two sets of, conformations corresponding to the cis and trans conformations of Pro31., The structure of SHL-I also has high similarity with that of the, N-terminus of hevein, a lectin from rubber-tree latex.
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<StructureSection load='1qk7' size='340' side='right'caption='[[1qk7]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qk7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyriopagopus_schmidti Cyriopagopus schmidti]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QK7 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qk7 OCA], [https://pdbe.org/1qk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qk7 RCSB], [https://www.ebi.ac.uk/pdbsum/1qk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qk7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TXLA1_CYRSC TXLA1_CYRSC] Agglutinates human and mice erythrocytes. This activity can be specifically inhibited by mannosamine. This lectin shows very low toxicity in both mammals and insects.<ref>PMID:8588212</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three-dimensional structure of native SHL-I, a lectin from the venom of the Chinese bird spider Selenocosmia huwena, has been determined from two-dimensional 1H NMR spectroscopy recorded at 500 and 600 MHz. The best 10 structures have NOE violation &lt;0.3 A, dihedral violation &lt;2 deg, and average root-mean-square differences of 0.85 + 0.06 A over backbone atoms. The structure consists of a three-stranded antiparallel beta-sheet and three turns. The three disulfide bridges and three-stranded antiparallel beta-sheet form a inhibitor cystine knot motif which is adopted by several other small proteins, such as huwentoxin-I, omega-conotoxin, and gurmarin. The C-terminal fragment from Leu28 to Trp32 adopts two sets of conformations corresponding to the cis and trans conformations of Pro31. The structure of SHL-I also has high similarity with that of the N-terminus of hevein, a lectin from rubber-tree latex.
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==About this Structure==
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Three-dimensional structure of Selenocosmia huwena lectin-I (SHL-I) from the venom of the spider Selenocosmia huwena by 2D-NMR.,Lu S, Liang S, Gu X J Protein Chem. 1999 Jul;18(5):609-17. PMID:10524779<ref>PMID:10524779</ref>
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1QK7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QK7 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Three-dimensional structure of Selenocosmia huwena lectin-I (SHL-I) from the venom of the spider Selenocosmia huwena by 2D-NMR., Lu S, Liang S, Gu X, J Protein Chem. 1999 Jul;18(5):609-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10524779 10524779]
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</div>
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[[Category: Ornithoctonus huwena]]
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<div class="pdbe-citations 1qk7" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Gu, X.]]
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<references/>
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[[Category: Liang, S.]]
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__TOC__
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[[Category: Lu, S.]]
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</StructureSection>
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[[Category: cystine knot]]
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[[Category: Cyriopagopus schmidti]]
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[[Category: lectin]]
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[[Category: Large Structures]]
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[[Category: selenocosmia huwena]]
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[[Category: Gu X]]
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[[Category: shl-i]]
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[[Category: Liang S]]
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[[Category: Lu S]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:46:01 2007''
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Current revision

Solution structure of Selenocosmia huwena lectin-I(SHL-I) by 2D-NMR

PDB ID 1qk7

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