2gwl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:59, 5 May 2021) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2gwl.png|left|200px]]
 
-
<!--
+
==Crystal structure of the Salmonella SpvB ATR Domain in complex with NADH==
-
The line below this paragraph, containing "STRUCTURE_2gwl", creates the "Structure Box" on the page.
+
<StructureSection load='2gwl' size='340' side='right'caption='[[2gwl]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2gwl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GWL FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAI:1,4-DIHYDRONICOTINAMIDE+ADENINE+DINUCLEOTIDE'>NAI</scene></td></tr>
-
-->
+
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2gwm|2gwm]]</div></td></tr>
-
{{STRUCTURE_2gwl| PDB=2gwl | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gwl OCA], [https://pdbe.org/2gwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gwl RCSB], [https://www.ebi.ac.uk/pdbsum/2gwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gwl ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[[https://www.uniprot.org/uniprot/SPVB_SALCH SPVB_SALCH]] Mono-ADP-ribosylates eukaryotic muscle and non-muscle actin on 'Arg-177'. ADP-ribosylation prevents the polymerization of G-actin to F-actin, causing actin filament depolymerization, destruction of the cytoskeleton and cytotoxicity. Does not possess NAD(+)-glycohydrolase activity, unlike most mART enzymes (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gw/2gwl_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gwl ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts.
-
===Crystal structure of the Salmonella SpvB ATR Domain in complex with NADH===
+
A steric antagonism of actin polymerization by a salmonella virulence protein.,Margarit SM, Davidson W, Frego L, Stebbins CE Structure. 2006 Aug;14(8):1219-29. PMID:16905096<ref>PMID:16905096</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_16905096}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2gwl" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 16905096 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16905096}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
2GWL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GWL OCA].
+
[[Category: Margarit, S M]]
-
 
+
[[Category: Stebbins, C E]]
-
==Reference==
+
-
A steric antagonism of actin polymerization by a salmonella virulence protein., Margarit SM, Davidson W, Frego L, Stebbins CE, Structure. 2006 Aug;14(8):1219-29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16905096 16905096]
+
-
[[Category: Salmonella typhimurium]]
+
-
[[Category: Single protein]]
+
-
[[Category: Margarit, S M.]]
+
-
[[Category: Stebbins, C E.]]
+
[[Category: Adp-ribosyltransferase]]
[[Category: Adp-ribosyltransferase]]
[[Category: Salmonella]]
[[Category: Salmonella]]
[[Category: Spvb]]
[[Category: Spvb]]
-
 
+
[[Category: Transferase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:05:25 2008''
+

Current revision

Crystal structure of the Salmonella SpvB ATR Domain in complex with NADH

PDB ID 2gwl

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2gwl"
Personal tools