1qoj

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(New page: 200px<br /><applet load="1qoj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qoj, resolution 3.0&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1qoj.gif|left|200px]]<br /><applet load="1qoj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qoj, resolution 3.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF E.COLI UVRB C-TERMINAL DOMAIN, AND A MODEL FOR UVRB-UVRC INTERACTION.'''<br />
 
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==Overview==
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==Crystal Structure of E.coli UvrB C-terminal domain, and a model for UvrB-UvrC interaction.==
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A crystal structure of the C-terminal domain of Escherichia coli UvrB, (UvrB') has been solved to 3.0 A resolution. The domain adopts a, helix-loop-helix fold which is stabilised by the packing of hydrophobic, side-chains between helices. From the UvrB' fold, a model for a domain of, UvrC (UvrC') that has high sequence homology with UvrB' has been made. In, the crystal, a dimerisation of UvrB domains is seen involving specific, hydrophobic and salt bridge interactions between residues in and close to, the loop region of the domain. It is proposed that a homologous mode of, interaction may occur between UvrB and UvrC. This interaction is likely to, be flexible, potentially spanning &gt; 50 A.
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<StructureSection load='1qoj' size='340' side='right'caption='[[1qoj]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qoj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QOJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qoj OCA], [https://pdbe.org/1qoj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qoj RCSB], [https://www.ebi.ac.uk/pdbsum/1qoj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qoj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UVRB_ECOLI UVRB_ECOLI] The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.<ref>PMID:12145219</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qo/1qoj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qoj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A crystal structure of the C-terminal domain of Escherichia coli UvrB (UvrB') has been solved to 3.0 A resolution. The domain adopts a helix-loop-helix fold which is stabilised by the packing of hydrophobic side-chains between helices. From the UvrB' fold, a model for a domain of UvrC (UvrC') that has high sequence homology with UvrB' has been made. In the crystal, a dimerisation of UvrB domains is seen involving specific hydrophobic and salt bridge interactions between residues in and close to the loop region of the domain. It is proposed that a homologous mode of interaction may occur between UvrB and UvrC. This interaction is likely to be flexible, potentially spanning &gt; 50 A.
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==About this Structure==
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Crystal structure of Escherichia coli UvrB C-terminal domain, and a model for UvrB-uvrC interaction.,Sohi M, Alexandrovich A, Moolenaar G, Visse R, Goosen N, Vernede X, Fontecilla-Camps JC, Champness J, Sanderson MR FEBS Lett. 2000 Jan 14;465(2-3):161-4. PMID:10631326<ref>PMID:10631326</ref>
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1QOJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QOJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of Escherichia coli UvrB C-terminal domain, and a model for UvrB-uvrC interaction., Sohi M, Alexandrovich A, Moolenaar G, Visse R, Goosen N, Vernede X, Fontecilla-Camps JC, Champness J, Sanderson MR, FEBS Lett. 2000 Jan 14;465(2-3):161-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10631326 10631326]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 1qoj" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Alexandrovich, A.]]
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[[Category: Champness, J.]]
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[[Category: Fontecilla-Camps, J.]]
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[[Category: Goosen, N.]]
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[[Category: Moolenaar, G.]]
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[[Category: Sanderson, M.R.]]
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[[Category: Sohi, M.]]
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[[Category: Vernede, X.]]
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[[Category: Visse, R.]]
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[[Category: nucleotide excision repair]]
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[[Category: uvrb protein]]
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[[Category: uvrb-c interaction]]
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[[Category: x-ray crystallography]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:50:51 2007''
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==See Also==
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*[[UvrABC 3D structures|UvrABC 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Alexandrovich A]]
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[[Category: Champness J]]
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[[Category: Fontecilla-Camps J]]
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[[Category: Goosen N]]
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[[Category: Moolenaar G]]
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[[Category: Sanderson MR]]
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[[Category: Sohi M]]
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[[Category: Vernede X]]
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[[Category: Visse R]]

Current revision

Crystal Structure of E.coli UvrB C-terminal domain, and a model for UvrB-UvrC interaction.

PDB ID 1qoj

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