This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qp2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1qp2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qp2" /> '''SOLUTION STRUCTURE OF PHOTOSYSTEM I ACCESSOR...)
Current revision (06:06, 17 April 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1qp2.jpg|left|200px]]<br /><applet load="1qp2" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1qp2" />
 
-
'''SOLUTION STRUCTURE OF PHOTOSYSTEM I ACCESSORY PROTEIN E FROM THE CYANOBACTERIUM NOSTOC SP. STRAIN PCC 8009'''<br />
 
-
==Overview==
+
==SOLUTION STRUCTURE OF PHOTOSYSTEM I ACCESSORY PROTEIN E FROM THE CYANOBACTERIUM NOSTOC SP. STRAIN PCC 8009==
-
PsaE is a small basic subunit located on the stromal (cytoplasmic) side of, photosystem I. In cyanobacteria, this subunit participates in cyclic, electron transport and modulates the interactions of the complex with, soluble ferredoxin. The PsaE protein isolated from the cyanobacterium, Synechococcus sp. strain PCC 7002 adopts the beta topology of an SH3, domain, with five beta strands (betaA through betaE) and a turn of 3(10), helix between strands betaD and betaE [Falzone, C. J., Kao, Y.-H., Zhao, J., Bryant, D. A., and Lecomte, J. T. J. (1994) Biochemistry 33, 6052-6062]. The primary structure of the PsaE protein is strongly, conserved across all oxygen-evolving photosynthetic organisms. However, variability in loop lengths, as well as N- or C-terminal extensions, suggests that the structure of a second representative PsaE subunit would, be useful to characterize the interactions among photosystem I, polypeptides. In this work, the solution structure of PsaE from the, cyanobacterium Nostoc sp. strain PCC 8009 was determined by NMR methods., Compared to PsaE from Synechococcus sp. strain PCC 7002, this PsaE has a, seven-residue deletion in the loop connecting strands betaC and betaD, and, an eight-residue C-terminal extension. Angular and distance restraints, derived from homonuclear and heteronuclear NMR experiments were used to, calculate structures by a distance-geometry/simulated-annealing protocol., A family of 20 structures (rmsd of 0.24 A in the regular secondary, structure) is presented. Differences between the two cyanobacterial, proteins are mostly confined to the CD loop region; the C-terminal, extension is disordered. The thermodynamic stability of Nostoc sp. strain, PCC 8009 PsaE toward urea denaturation was measured by circular dichroism, and fluorescence spectroscopy, and thermal denaturation was monitored by, UV absorption spectroscopy. Chemical and thermal denaturation curves are, modeled satisfactorily with two-state processes. The DeltaG degrees of, unfolding at room temperature is 12.4 +/- 0.3 kJ mol(-1) (pH 5), and the, thermal transition midpoint is 59 +/- 1 degrees C (pH 7). Interactions, with other proteins in the photosystem I complex may aid in maintaining, PsaE in its native state under physiological conditions.
+
<StructureSection load='1qp2' size='340' side='right'caption='[[1qp2]]' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1qp2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_8009 Nostoc sp. PCC 8009]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QP2 FirstGlance]. <br>
-
1QP2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_sp. Nostoc sp.]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QP2 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qp2 OCA], [https://pdbe.org/1qp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qp2 RCSB], [https://www.ebi.ac.uk/pdbsum/1qp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qp2 ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
The solution structure of photosystem I accessory protein E from the cyanobacterium Nostoc sp. strain PCC 8009., Mayer KL, Shen G, Bryant DA, Lecomte JT, Falzone CJ, Biochemistry. 1999 Oct 12;38(41):13736-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10521281 10521281]
+
== Function ==
-
[[Category: Nostoc sp.]]
+
[https://www.uniprot.org/uniprot/PSAE_NOSS8 PSAE_NOSS8] Stabilizes the interaction between PsaC and the PSI core, assists the docking of the ferredoxin to PSI and interacts with ferredoxin-NADP oxidoreductase.
-
[[Category: Single protein]]
+
== Evolutionary Conservation ==
-
[[Category: Bryant, D.A.]]
+
[[Image:Consurf_key_small.gif|200px|right]]
-
[[Category: Falzone, C.J.]]
+
Check<jmol>
-
[[Category: Lecomte, J.T.J.]]
+
<jmolCheckbox>
-
[[Category: Mayer, K.L.]]
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/1qp2_consurf.spt"</scriptWhenChecked>
-
[[Category: Shen, G.]]
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
[[Category: mainly beta]]
+
<text>to colour the structure by Evolutionary Conservation</text>
-
[[Category: pleckstrin topology]]
+
</jmolCheckbox>
-
[[Category: roll]]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qp2 ConSurf].
-
[[Category: sh3-like]]
+
<div style="clear:both"></div>
-
 
+
__TOC__
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:51:29 2007''
+
</StructureSection>
 +
[[Category: Large Structures]]
 +
[[Category: Nostoc sp. PCC 8009]]
 +
[[Category: Bryant DA]]
 +
[[Category: Falzone CJ]]
 +
[[Category: Lecomte JTJ]]
 +
[[Category: Mayer KL]]
 +
[[Category: Shen G]]

Current revision

SOLUTION STRUCTURE OF PHOTOSYSTEM I ACCESSORY PROTEIN E FROM THE CYANOBACTERIUM NOSTOC SP. STRAIN PCC 8009

PDB ID 1qp2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qp2"
Personal tools