This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1qpp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1qpp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qpp, resolution 2.6&Aring;" /> '''CRYSTAL STRUCTURES OF...)
Current revision (06:06, 17 April 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1qpp.gif|left|200px]]<br /><applet load="1qpp" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1qpp, resolution 2.6&Aring;" />
 
-
'''CRYSTAL STRUCTURES OF SELF CAPPING PAPD CHAPERONE HOMODIMERS'''<br />
 
-
==Overview==
+
==CRYSTAL STRUCTURES OF SELF CAPPING PAPD CHAPERONE HOMODIMERS==
-
PapD is an immunoglobulin-like chaperone that mediates the assembly of P, pili in uropathogenic strains of Escherichia coli. It binds and caps, interactive surfaces on pilus subunits to prevent their premature, associations in the periplasm. We elucidated the structural basis of a, mechanism whereby PapD also interacts with itself, capping its own subunit, binding surface. Crystal structures of dimeric forms of PapD revealed that, this self-capping mechanism involves a rearrangement and ordering of the, C2-D2 and F1-G1 loops upon dimerization which might ensure that a stable, dimer is not formed in solution in spite of a relatively large dimer, interface. An analysis of site directed mutations revealed that chaperone, dimerization requires the same surface that is otherwise used to bind, subunits.
+
<StructureSection load='1qpp' size='340' side='right'caption='[[1qpp]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1qpp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QPP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QPP FirstGlance]. <br>
-
1QPP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QPP OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qpp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qpp OCA], [https://pdbe.org/1qpp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qpp RCSB], [https://www.ebi.ac.uk/pdbsum/1qpp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qpp ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Structural basis of chaperone self-capping in P pilus biogenesis., Hung DL, Pinkner JS, Knight SD, Hultgren SJ, Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8178-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10393968 10393968]
+
== Function ==
 +
[https://www.uniprot.org/uniprot/PAPD_ECOLX PAPD_ECOLX] Binds and caps interactive surfaces on pilus subunits to prevent them from participating in non-productive interactions. Facilitates the import of subunits into the periplasm. May facilitate subunit folding. Chaperone-subunit complexes are then targeted to the PapC outer membrane usher where the chaperone must uncap from the subunits.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qp/1qpp_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qpp ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Hultgren, S.J.]]
+
[[Category: Hultgren SJ]]
-
[[Category: Hung, D.L.]]
+
[[Category: Hung DL]]
-
[[Category: Knight, S.D.]]
+
[[Category: Knight SD]]
-
[[Category: Pinkner, J.S.]]
+
[[Category: Pinkner JS]]
-
[[Category: beta barrel]]
+
-
[[Category: immunoglobulin fold chaperone]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:52:20 2007''
+

Current revision

CRYSTAL STRUCTURES OF SELF CAPPING PAPD CHAPERONE HOMODIMERS

PDB ID 1qpp

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qpp"
Personal tools