1qr4

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(New page: 200px<br /><applet load="1qr4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qr4, resolution 2.55&Aring;" /> '''TWO FIBRONECTIN TYPE...)
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[[Image:1qr4.gif|left|200px]]<br /><applet load="1qr4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qr4, resolution 2.55&Aring;" />
 
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'''TWO FIBRONECTIN TYPE-III DOMAIN SEGMENT FROM CHICKEN TENASCIN'''<br />
 
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==Overview==
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==TWO FIBRONECTIN TYPE-III DOMAIN SEGMENT FROM CHICKEN TENASCIN==
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A fragment of chicken tenascin consisting of fibronectin type-III domains, 5 and 6 has been expressed in Escherichia coli. After modifying a, previously reported purification protocol, an electrophoretically, homogeneous recombinant protein was obtained from which various crystal, forms could be grown under identical conditions. Only one form was, suitable for structure determination. These crystals belong to space group, P21, with unit-cell parameters a = 45.2, b = 57.9, c = 72.2 A, beta = 91.4, degrees, and diffract to at least 2.6 A resolution using synchrotron, radiation. From density measurements of the crystals, it was found that, there are two molecules in the asymmetric unit. Diffraction data of, native, two platinum-derivative and one palladium-derivative crystals were, collected.
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<StructureSection load='1qr4' size='340' side='right'caption='[[1qr4]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qr4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QR4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qr4 OCA], [https://pdbe.org/1qr4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qr4 RCSB], [https://www.ebi.ac.uk/pdbsum/1qr4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qr4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TENA_CHICK TENA_CHICK] Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qr/1qr4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qr4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A fragment of chicken tenascin consisting of fibronectin type-III domains 5 and 6 has been expressed in Escherichia coli. After modifying a previously reported purification protocol, an electrophoretically homogeneous recombinant protein was obtained from which various crystal forms could be grown under identical conditions. Only one form was suitable for structure determination. These crystals belong to space group P21, with unit-cell parameters a = 45.2, b = 57.9, c = 72.2 A, beta = 91.4 degrees, and diffract to at least 2.6 A resolution using synchrotron radiation. From density measurements of the crystals, it was found that there are two molecules in the asymmetric unit. Diffraction data of native, two platinum-derivative and one palladium-derivative crystals were collected.
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==About this Structure==
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Purification, crystallization and preliminary crystallographic studies of a two fibronectin type-III domain segment from chicken tenascin encompassing the heparin- and contactin-binding regions.,Bisig D, Weber P, Vaughan L, Winterhalter KH, Piontek K Acta Crystallogr D Biol Crystallogr. 1999 May;55(Pt 5):1069-73. PMID:10216309<ref>PMID:10216309</ref>
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1QR4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QR4 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Purification, crystallization and preliminary crystallographic studies of a two fibronectin type-III domain segment from chicken tenascin encompassing the heparin- and contactin-binding regions., Bisig D, Weber P, Vaughan L, Winterhalter KH, Piontek K, Acta Crystallogr D Biol Crystallogr. 1999 May;55(Pt 5):1069-73. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10216309 10216309]
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</div>
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<div class="pdbe-citations 1qr4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bisig, D.A.]]
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[[Category: Bisig DA]]
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[[Category: Piontek, K.]]
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[[Category: Piontek K]]
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[[Category: adhesion]]
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[[Category: extracellular matrix]]
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[[Category: fibronectin type-iii]]
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[[Category: fusion protein]]
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[[Category: heparin]]
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[[Category: tenascin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:54:38 2007''
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Current revision

TWO FIBRONECTIN TYPE-III DOMAIN SEGMENT FROM CHICKEN TENASCIN

PDB ID 1qr4

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