1qsn

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(New page: 200px<br /><applet load="1qsn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qsn, resolution 2.20&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1qsn.gif|left|200px]]<br /><applet load="1qsn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qsn, resolution 2.20&Aring;" />
 
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'''CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND COENZYME A AND HISTONE H3 PEPTIDE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND COENZYME A AND HISTONE H3 PEPTIDE==
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Gene activation is a highly regulated process that requires the, coordinated action of proteins to relieve chromatin repression and to, promote transcriptional activation. Nuclear histone acetyltransferase, (HAT) enzymes provide a mechanistic link between chromatin destabilization, and gene activation by acetylating the epsilon-amino group of specific, lysine residues within the aminoterminal tails of core histones to, facilitate access to DNA by transcriptional activators. Here we report the, high-resolution crystal structure of the HAT domain of Tetrahymena GCN5, (tGCN5) bound with both its physiologically relevant ligands, coenzyme A, (CoA) and a histone H3 peptide, and the structures of nascent tGCN5 and a, tGCN5/acetyl-CoA complex. Our structural data reveal histone-binding, specificity for a random-coil structure containing a G-K-X-P recognition, sequence, and show that CoA is essential for reorienting the enzyme for, histone binding. Catalysis appears to involve water-mediated proton, extraction from the substrate lysine by a glutamic acid general base and a, backbone amide that stabilizes the transition-state reaction intermediate., Comparison with related N-acetyltransferases indicates a conserved, structural framework for CoA binding and catalysis, and structural, variability in regions associated with substrate-specific binding.
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<StructureSection load='1qsn' size='340' side='right'caption='[[1qsn]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1qsn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QSN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QSN FirstGlance]. <br>
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1QSN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] and [http://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila] with COA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QSN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qsn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qsn OCA], [https://pdbe.org/1qsn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qsn RCSB], [https://www.ebi.ac.uk/pdbsum/1qsn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qsn ProSAT]</span></td></tr>
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Structure of Tetrahymena GCN5 bound to coenzyme A and a histone H3 peptide., Rojas JR, Trievel RC, Zhou J, Mo Y, Li X, Berger SL, Allis CD, Marmorstein R, Nature. 1999 Sep 2;401(6748):93-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10485713 10485713]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[https://www.uniprot.org/uniprot/Q27198_TETTH Q27198_TETTH]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qs/1qsn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qsn ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Tetrahymena thermophila]]
[[Category: Tetrahymena thermophila]]
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[[Category: Allis, C.David.]]
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[[Category: Berger SL]]
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[[Category: Berger, S.L.]]
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[[Category: David Allis C]]
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[[Category: Li, X.]]
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[[Category: Li X]]
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[[Category: Marmorstein, R.]]
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[[Category: Marmorstein R]]
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[[Category: Mo, Y.]]
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[[Category: Mo Y]]
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[[Category: Rojas, J.R.]]
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[[Category: Rojas JR]]
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[[Category: Trievel, R.C.]]
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[[Category: Trievel RC]]
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[[Category: Zhou, J.]]
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[[Category: Zhou J]]
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[[Category: COA]]
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[[Category: coa-binding protein]]
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[[Category: gcn5-related n-acetyltransferase]]
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[[Category: histone acetyltransferase]]
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[[Category: ternary complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 00:56:56 2007''
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Current revision

CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND COENZYME A AND HISTONE H3 PEPTIDE

PDB ID 1qsn

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