1qwq

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(New page: 200px<br /><applet load="1qwq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qwq" /> '''Solution structure of the monomeric N67D mut...)
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[[Image:1qwq.jpg|left|200px]]<br /><applet load="1qwq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qwq" />
 
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'''Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease'''<br />
 
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==Overview==
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==Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease==
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Bovine seminal ribonuclease (BS-RNase), the only dimeric protein among the, pancreatic-like ribonucleases, is endowed with special structural features, and with biological functions beyond enzymatic activity. In solution, the, protein exists as an equilibrium mixture of two forms, with or without, exchange (or swapping) of the N-terminal arms. After selective reduction, and alkylation of the two intrachain disulfide bridges, the dimeric, protein can be transformed into a monomeric derivative that has a, ribonuclease activity higher than that of the parent dimeric protein but, is devoid of the special biological functions. A detailed investigation of, the structural features of this protein in solution, in comparison with, those of other monomeric ribonucleases, may help unveil the structural, details which induce swapping of the N-terminal arms of BS-RNase. The, solution structure of the recombinant monomeric form of BS-RNase, as, determined by 3D heteronuclear NMR, shows close similarity with that of, bovine pancreatic ribonuclease (RNase A) in all regions characterized by, regular elements of secondary structure. However, significant differences, are present in the flexible regions, which could account for the different, behavior of the two proteins. To characterize in detail these regions, we, have measured H/D exchange rate constants, temperature coefficients and, heteronuclear NOEs of backbone amides for both RNase A and monomeric, BS-RNase. The results indicate a large difference in the backbone, flexibility of the hinge peptide segment 16-22 of the two proteins, which, could provide the molecular basis to explain the ability of BS-RNase, subunits to swap their N-terminal arms.
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<StructureSection load='1qwq' size='340' side='right'caption='[[1qwq]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [https://pdbe.org/1qwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [https://www.ebi.ac.uk/pdbsum/1qwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNS_BOVIN RNS_BOVIN] This enzyme hydrolyzes both single- and double-stranded RNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qwq ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1QWQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure superseeds the now removed PDB entry 1O0G. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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The swapping of terminal arms in ribonucleases: comparison of the solution structure of monomeric bovine seminal and pancreatic ribonucleases., Avitabile F, Alfano C, Spadaccini R, Crescenzi O, D'Ursi AM, D'Alessio G, Tancredi T, Picone D, Biochemistry. 2003 Jul 29;42(29):8704-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12873130 12873130]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Alfano C]]
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[[Category: Alessio, G.D.]]
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[[Category: Avitabile F]]
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[[Category: Alfano, C.]]
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[[Category: Crescenzi O]]
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[[Category: Avitabile, F.]]
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[[Category: D'Alessio G]]
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[[Category: Crescenzi, O.]]
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[[Category: D'Ursi AM]]
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[[Category: Picone, D.]]
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[[Category: Picone D]]
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[[Category: Spadaccini, R.]]
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[[Category: Spadaccini R]]
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[[Category: Tancredi, T.]]
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[[Category: Tancredi T]]
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[[Category: Ursi, A.M.D.]]
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[[Category: alpha-beta-protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:04:23 2007''
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Current revision

Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease

PDB ID 1qwq

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