3dif

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{{Seed}}
 
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[[Image:3dif.jpg|left|200px]]
 
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==Crystal structure of FabOX117==
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The line below this paragraph, containing "STRUCTURE_3dif", creates the "Structure Box" on the page.
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<StructureSection load='3dif' size='340' side='right'caption='[[3dif]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3dif]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DIF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3dif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dif OCA], [https://pdbe.org/3dif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3dif RCSB], [https://www.ebi.ac.uk/pdbsum/3dif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3dif ProSAT]</span></td></tr>
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{{STRUCTURE_3dif| PDB=3dif | SCENE= }}
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/di/3dif_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3dif ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We describe a pipeline for the rapid production of recombinant Fabs derived from mouse monoclonal antibodies suitable for use in structural studies. The pipeline is exemplified by the production of three Fabs derived from the monoclonal antibodies OX108 (anti-CD200 receptor), OX117 and OX119 (anti-SIRPgamma). Heavy and light chain variable domains were inserted into separate expression vectors containing resident constant regions using In-Fusion PCR cloning. Following transient co-expression in HEK 293T cells, secreted Fab fragments were purified by metal chelate chromatography and gel filtration using an automated procedure with yields of up to 4mg/L of cell culture. Following crystallization trials, diffracting crystals were obtained for the recombinant Fabs of OX108 and OX117, and their structures solved to 2.3A and 2.4A, respectively.
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===Crystal structure of FabOX117===
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A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells.,Nettleship JE, Ren J, Rahman N, Berrow NS, Hatherley D, Barclay AN, Owens RJ Protein Expr Purif. 2008 Nov;62(1):83-9. Epub 2008 Jul 10. PMID:18662785<ref>PMID:18662785</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3dif" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18662785}}, adds the Publication Abstract to the page
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*[[Antibody 3D structures|Antibody 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18662785 is the PubMed ID number.
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*[[Sandbox 20009|Sandbox 20009]]
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*[[3D structures of human antibody|3D structures of human antibody]]
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{{ABSTRACT_PUBMED_18662785}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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3DIF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DIF OCA].
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</StructureSection>
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==Reference==
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A pipeline for the production of antibody fragments for structural studies using transient expression in HEK 293T cells., Nettleship JE, Ren J, Rahman N, Berrow NS, Hatherley D, Neil Barclay A, Owens RJ, Protein Expr Purif. 2008 Jul 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18662785 18662785]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Nettleship, J E.]]
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[[Category: Nettleship JE]]
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[[Category: OPPF, Oxford Protein Production Facility.]]
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[[Category: Owens RJ]]
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[[Category: Owens, R J.]]
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[[Category: Ren J]]
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[[Category: Ren, J.]]
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[[Category: Antibody fragment]]
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[[Category: Fab]]
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[[Category: Immune system]]
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[[Category: Immunoglobulin domain]]
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[[Category: Oppf]]
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[[Category: Oxford protein production facility]]
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[[Category: Structural genomic]]
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[[Category: Transient expression]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Aug 13 13:49:49 2008''
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Current revision

Crystal structure of FabOX117

PDB ID 3dif

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