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1qz6

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(New page: 200px<br /><applet load="1qz6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qz6, resolution 1.60&Aring;" /> '''Structure of rabbit ...)
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[[Image:1qz6.jpg|left|200px]]<br /><applet load="1qz6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1qz6, resolution 1.60&Aring;" />
 
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'''Structure of rabbit actin in complex with jaspisamide A'''<br />
 
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==Overview==
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==Structure of rabbit actin in complex with jaspisamide A==
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Marine macrolide toxins of trisoxazole family target actin with high, affinity and specificity and have promising pharmacological properties. We, present X-ray structures of actin in complex with two members of this, family, kabiramide C and jaspisamide A, at a resolution of 1.45 and 1.6 A, respectively. The structures reveal the absolute stereochemistry of these, toxins and demonstrate that their trisoxazole ring interacts with actin, subdomain 1 while the aliphatic side chain is inserted into the, hydrophobic cavity between actin subdomains 1 and 3. The binding site is, essentially the same as the one occupied by the actin-capping domain of, the gelsolin superfamily of proteins. The structural evidence suggests, that actin filament severing and capping by these toxins is also analogous, to that of gelsolin. Consequently, these macrolides may be viewed as small, molecule biomimetics of an entire class of actin-binding proteins.
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<StructureSection load='1qz6' size='340' side='right'caption='[[1qz6]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1qz6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZ6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=JAS:JASPISAMIDE+A'>JAS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qz6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qz6 OCA], [https://pdbe.org/1qz6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qz6 RCSB], [https://www.ebi.ac.uk/pdbsum/1qz6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qz6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Marine macrolide toxins of trisoxazole family target actin with high affinity and specificity and have promising pharmacological properties. We present X-ray structures of actin in complex with two members of this family, kabiramide C and jaspisamide A, at a resolution of 1.45 and 1.6 A, respectively. The structures reveal the absolute stereochemistry of these toxins and demonstrate that their trisoxazole ring interacts with actin subdomain 1 while the aliphatic side chain is inserted into the hydrophobic cavity between actin subdomains 1 and 3. The binding site is essentially the same as the one occupied by the actin-capping domain of the gelsolin superfamily of proteins. The structural evidence suggests that actin filament severing and capping by these toxins is also analogous to that of gelsolin. Consequently, these macrolides may be viewed as small molecule biomimetics of an entire class of actin-binding proteins.
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==About this Structure==
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Trisoxazole macrolide toxins mimic the binding of actin-capping proteins to actin.,Klenchin VA, Allingham JS, King R, Tanaka J, Marriott G, Rayment I Nat Struct Biol. 2003 Dec;10(12):1058-63. Epub 2003 Oct 26. PMID:14578936<ref>PMID:14578936</ref>
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1QZ6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with SUC, CA, ATP and JAS as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1QZ6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Trisoxazole macrolide toxins mimic the binding of actin-capping proteins to actin., Klenchin VA, Allingham JS, King R, Tanaka J, Marriott G, Rayment I, Nat Struct Biol. 2003 Dec;10(12):1058-63. Epub 2003 Oct 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14578936 14578936]
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</div>
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[[Category: Oryctolagus cuniculus]]
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<div class="pdbe-citations 1qz6" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Allingham, J.S.]]
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[[Category: King, R.]]
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[[Category: Klenchin, V.A.]]
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[[Category: Marriott, G.]]
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[[Category: Rayment, I.]]
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[[Category: Tanaka, J.]]
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[[Category: ATP]]
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[[Category: CA]]
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[[Category: JAS]]
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[[Category: SUC]]
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[[Category: actin]]
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[[Category: jaspisamide a]]
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[[Category: toxin]]
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[[Category: trisoxazole]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:08:29 2007''
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==See Also==
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*[[Actin 3D structures|Actin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Oryctolagus cuniculus]]
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[[Category: Allingham JS]]
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[[Category: King R]]
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[[Category: Klenchin VA]]
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[[Category: Marriott G]]
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[[Category: Rayment I]]
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[[Category: Tanaka J]]

Current revision

Structure of rabbit actin in complex with jaspisamide A

PDB ID 1qz6

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