1r0q

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(New page: 200px<br /><applet load="1r0q" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r0q, resolution 1.61&Aring;" /> '''Characterization of ...)
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[[Image:1r0q.gif|left|200px]]<br /><applet load="1r0q" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1r0q, resolution 1.61&Aring;" />
 
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'''Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (Spirographis) heme'''<br />
 
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==Overview==
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==Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (Spirographis) heme==
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Expression of the truncated (lacking an N-terminal signal sequence), structural gene of Thermus thermophilus cytochrome c(552) in the cytoplasm, of Escherichia coli yields both dimeric (rC(557)) and monomeric (rC(552)), cytochrome c-like proteins [Keightley, J. A., et al. (1998) J. Biol. Chem., 273, 12006-12016], which form spontaneously without the involvement of, cytochrome c maturation factors. Cytochrome rC(557) is comprised of a, dimer and has been structurally characterized [McRee, D., et al. (2001) J., Biol. Chem. 276, 6537-6544]. Unexpectedly, the monomeric rC(552), transforms spontaneously to a cytochrome-like chromophore having, in its, reduced state, the Q(oo) transition (alpha-band) at 572 nm (therefore, called p572). The X-ray crystallographic structure of rC(552), at 1.41 A, resolution, shows that the 2-vinyl group of heme ring I is converted to a, [heme-CO-CH(2)-S-CH(2)-C(alpha)] conjugate with cysteine 11. Electron, density maps obtained from isomorphous crystals of p572 at 1.61 A, resolution reveal that the 2-vinyl group has been oxidized to a formyl, group. This explains the lower energy of the Q(oo)() transition, the, presence of a new, high-frequency band in the resonance Raman spectra at, 1666 cm(-1) for oxidized and at 1646 cm(-1) for reduced samples, and the, greatly altered, paramagnetically shifted (1)H NMR spectrum observed for, this species. The overall process defines a novel mechanism for oxidation, of the 2-vinyl group to a 2-formyl group and adds to the surprising array, of chemical reactions that occur in the interaction of heme with the CXXCH, sequence motif in apocytochromes c.
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<StructureSection load='1r0q' size='340' side='right'caption='[[1r0q]], [[Resolution|resolution]] 1.61&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1r0q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R0Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R0Q FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HFM:2-FORMYL-PROTOPORPHRYN+IX'>HFM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r0q OCA], [https://pdbe.org/1r0q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r0q RCSB], [https://www.ebi.ac.uk/pdbsum/1r0q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r0q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CY552_THETH CY552_THETH] This monoheme basic protein appears to function as an electron donor to cytochrome oxidase in T.thermophilus.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r0/1r0q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r0q ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1R0Q is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with HFM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R0Q OCA].
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*[[Cytochrome c nitrite reductase|Cytochrome c nitrite reductase]]
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__TOC__
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==Reference==
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</StructureSection>
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Cytochrome rC552, formed during expression of the truncated, Thermus thermophilus cytochrome c552 gene in the cytoplasm of Escherichia coli, reacts spontaneously to form protein-bound 2-formyl-4-vinyl (Spirographis) heme., Fee JA, Todaro TR, Luna E, Sanders D, Hunsicker-Wang LM, Patel KM, Bren KL, Gomez-Moran E, Hill MG, Ai J, Loehr TM, Oertling WA, Williams PA, Stout CD, McRee D, Pastuszyn A, Biochemistry. 2004 Sep 28;43(38):12162-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15379555 15379555]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Thermus thermophilus]]
[[Category: Thermus thermophilus]]
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[[Category: Ai, J.]]
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[[Category: Ai J]]
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[[Category: Bren, K.L.]]
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[[Category: Bren KL]]
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[[Category: Fee, J.A.]]
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[[Category: Fee JA]]
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[[Category: Gomez-Moran, E.]]
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[[Category: Gomez-Moran E]]
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[[Category: Hill, M.G.]]
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[[Category: Hill MG]]
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[[Category: Hunsicker-Wang, L.M.]]
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[[Category: Hunsicker-Wang LM]]
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[[Category: Loehr, T.M.]]
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[[Category: Loehr TM]]
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[[Category: Luna, E.]]
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[[Category: Luna E]]
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[[Category: McRee, D.]]
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[[Category: McRee D]]
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[[Category: Oertling, W.A.]]
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[[Category: Oertling WA]]
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[[Category: Pastuszyn, A.]]
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[[Category: Pastuszyn A]]
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[[Category: Patel, K.M.]]
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[[Category: Patel KM]]
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[[Category: Sanders, D.]]
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[[Category: Sanders D]]
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[[Category: Stout, C.D.]]
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[[Category: Stout CD]]
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[[Category: Todaro, T.R.]]
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[[Category: Todaro TR]]
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[[Category: Williams, P.A.]]
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[[Category: Williams PA]]
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[[Category: HFM]]
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[[Category: malformed cytochrome c]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:10:14 2007''
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Current revision

Characterization of the conversion of the malformed, recombinant cytochrome rc552 to a 2-formyl-4-vinyl (Spirographis) heme

PDB ID 1r0q

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